Page 30 - Gates-AnnualReport-2016

A brief description of these core facilities and graphic summaries illustrating their use by both Gates Center members and nonmembers follows.
1
FLOW CYTOMETRY CORE
Flow cytometry (FC) is an essential tool for stem cell research. FC uses fluorescent tags in a laser-based fluidic instrument to quickly examine and collect cells at the single-cell level. By using multiple different fluorescent tags, the FC Core can identify the type of cells present in a sample and the biological status of these cells. The FC Core provides cutting-edge equipment at a cost-effective price, a highly enthusiastic and skilled staff, experiment design consultation, data analysis assistance and instrument training services to Gates members.
6000
4000
2000
0
Cell Sorting
ViCell Counts Analysis & Luminex
FLOW CYTOMETER CORE USE
24000
20000
16000
12000
8000 4000 0
Blocks Processed Paraffin Slides Frozen Slides H&E Staining
2012 2013
2014 2015 2016
2 MORPHOLOGY AND PHENOTYPING CORE
The ability of clinicians and scientists to analyze tissues at the microscopic level is essential for understanding disease mechanisms. To facilitate this, the Morphology and Phenotyping Core provides a full set of histology services. Upon consultations with the core’s histotechnicians, users submit tissue specimens that are processed according to the investigator’s needs. Tissue sections are prepared from the specimens to allow for a qualitative and quantitative analysis of the tissues. Analysis of these tissue sections using various staining procedures further contributes to understanding disease pathology.
MORPHOLOGY AND PHENOTYPING CORE USE
30 Gates Center for Regenerative Medicine
2011 2012
2013 2014
2015 2016