Page 36 - Gates-AnnualReport-2017

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FLOW CYTOMETRY CORE
Flow cytometry (FC) is an essential tool for stem cell research, allowing the examination of cells at the single-cell level by using cell surface, internal, and nuclear labels. We also have more specialized equipment that can rapidly isolate and collect unique types of cells.
Traditional flow cytometers use laser beams and fluorescent tags to identify the presence or absence of cell markers, however the number of easily identifiable labels is limited to 10 to 15 in conventional systems. Recently, with funding provided by the Gates Center and other campus sources, the core purchased a mass cytometer. This instrument uses rare-earth metal tags to easily identify up to 45 different markers on each cell.
The FC Core provides cutting-edge equipment at a cost-effective price, a highly enthusiastic and skilled staff, experiment design consultation, data analysis assistance, and instrument training services to Gates members.
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Cell Sorting Analysis & Luminex ViCell Counts Mass Cytometry
FLOW CYTOMETER CORE USE
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Blocks Processed Paraffin Slides Frozen Slides H&E Staining
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2 MORPHOLOGY AND PHENOTYPING CORE
The ability of clinicians and scientists to analyze tissues at the microscopic level is essential for understanding disease mechanisms. To facilitate this, the Morphology and Phenotyping Core provides a full set of histology services. Upon consultation with the core’s histotechnicians, users submit tissue specimens that are processed according to the investigator’s needs. Tissue sections are prepared from the specimens to allow for a qualitative and quantitative analysis of the tissues. Analysis of these tissue sections using various staining procedures further contributes to understanding disease pathology.
MORPHOLOGY AND PHENOTYPING CORE USE
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