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properties.Thesymbolsindicatetheaveragesfortheanalyzedcolonies，andtheerorbars indicatestandarddeviations.Theequivalentouterdiameterwas27土 15μmat370C，189土 77μmat400Cand359士 85μmat420C，respectively.Theatachedcirculardiameterwas 39土 56μmat370C，99土 37μmat400Cand154土 21μmat420C，respectively.The emptinesofcolonywas37土 15% at370C，52土 12% at400C and74士 8% at420C， respectively.The equivalent outer diameter，atached circular diameter and emptines increasedwiththeriseofthetemperature.
Table1PropertiesofL.pneumophilabiofilmmicrocoloniesincubatedat370C Position Numberof Outerdia.(μm) Atacheddia.(μm) Emptines(%)
colonies Average S.D. Average S.D. Average S.D. 。。。。。。。
Pl
P2 6 87 32 62 23 43 4 P3 18 138 81 81 25 43 18 P4 79 35 14 27 9 35 16
400
言 300 3
口 Outerdiam巴ter ロ Atacheddiameter ロ Emptines
Flowrate101];1
申*
r 100
ト80
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P司
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-r
。
吋~
() 200
ECロL
斗4 0 L司
40 同zoa
。 口 辺
ω
白 100
Incubationt BYE-αbroth N=103
Pl
巴
mp.37C
0
P2
Position
Fig.5Propertiesofιday-oldL.pneumophilabiofilmgrownonfour slidesinf10wchamberat370C.SymbolsofPvalueforトtestingare 牢 : P<0.05and牢牢 : P<0.01.
Fig.6CLMSappearanceofarepresentativebiofilmsectionformedonaglas slideof8-day-oldL.pneumophilabiofilm.Efectsofincubationtemperature on L. pneumophila biofilm properties under liquid f10w with BCYE目 α medium(A)370C;(B)40oC;(C)420C.
T
II
60 i'
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