Page 30 - Brochure Hibergene

15. Troubleshooting
Instructions for Use
Observation
Invalid result (no EC amplification)
Potential cause
Incorrect handling of sample during procedure
Presence of inhibitors in sample
Reaction mix not completely resuspended
Inadequate denaturation conditions
Incorrect volume of sample added
Reaction mixture not present at bottom of the tube after reconstitution
Use of expired materials
RNA/DNA contamination
Action
Repeat correct procedure
Repeat assay with a fresh sample
Repeat assay ensuring reaction mix is resuspended
Ensure dry bath incubator is set to 95°C and is at correct temperature
Check pipette calibration and repeat using correct volumes
Repeat assay ensuring that sample is at correct level after vortexing/centrifugation
Check expiry date and repeat testing with new kit if necessary
Decontaminate workspace and equipment using RNA/DNA cleaning solutions
IFU-024 Rev 03
Page 29 of 31
Signal in NTC