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McHugh , 2004 Walter , 2016)
Merlino 2013 Heat shocked 100 2 Polymerase chain reaction-denaturing gradient gel electrophoresis PCR-DGGE Real-time PCR Clostridium sp., Ruminococcaceae sp., Clostridiaceae cluster I, Clostridium sp., Clostridium sporosphaeroides, Clostridium cellulosi Thermoanaerobacterium sp Methanosarcina sp., Methanothermobacter sp., Methanosarcina mazeii Methanosaeta sp Firmicutes, Bacteroidetes Synergistes 7.3)
7.3 PCR-DGGE A B C Illumina amplicon sequencing Trace element
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