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pressure to the skin using the glass slide the blood in the capillary plexus can be 'squeezed' out. This has a major effect on transmission of the laser energy, particularly 532 nm, since less energy is absorbed in the blood layer. Hence, using a glass side on the skin surface during treatment with 532 nm will help to get more energy into deeper tattoo ink.
Location of the tattoo - clinical evidence suggests that certain parts of the body are easier to treat than others. I'm not aware of a proper clinical study to evaluate this but there is plenty of anecdotal evidence which backs up this claim. Distal tattoos, especially on the lower legs are definitely slower to respond, I have found. It may he better to leave slightly longer intervals between sessions on these areas.
The consequences of all of the above is that the choice of laser parameters and timings are very important in the treatment process. If you choose a 1064 nm wavelength, at 5 J/cm2 in a 5 mm spot with a pulsewidth of 10 ns, then you cannot expect the same result if you subsequently choose a 4 mm spot or 750 picoseconds or 694 nm or 8 J/cm2! Changing any one of these parameters will have an effect on the treatment efficiency. They are NOT equivalent so don't expect the same results.
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