Page 325 - AWSAR 2.0
P. 325

fluorophores like green fluorescent protein might get tagged but they hinder in the infectivity of the virus. Thus, need an alternative approach where the tag will be small, proteins will be visualised and sidewise
function will be retained. Here, an innovative approach has been taken for HA visualization and to track the movement from ER to membrane. A small tag called “tetracysteine”, four consecutive cysteine residues are incorporated which gives colour when incubated with FlAsH-EDT2. Fluorescent images show how HA production increases along with time and how it moves.
Identifying the host co-factors
There are some tags
which help in purification of proteins, like His
Ms. Oyahida Khatun || 301
tag, GST tag, etc. So if there is any interaction of other proteins with the tagged proteins then they will also be identified. The same approach is taken here also. A special tag called FLAG
   It is always fascinating to image any intracellular event at a molecular level. However, sometimes there are many obstructions. This is applicable here as well. Some common fluorophores like green fluorescent protein might get tagged but they hinder in the infectivity of the virus.
  other effective
tag is attached with HA and its interactors are also identified. A huge part of the interactors are host co-factors and they mainly reside in ER and golgi. So the new approach is to target proteins, reduce their expression by knocking down use of siRNA. Usual infectious period of influenza is around 3 to 4 days. So if the host factors are targeted to slow down their expression it will be harmful for the host but it will prevent spreading of influenza. So it might work as a drug target in an pandemic case where no options are available.
   
























































































   323   324   325   326   327