Page 34 - CSIR-IGIB Annual Report 2020-21

miRNAs. While only 17 miRNAs were found to its target genes, PDCD4 (programmed cell death
be differentially regulated between NL and L 4) and Maspin, both tumor-suppressors.
skin on day 1, as many as 129 miRNAs got Collectively, our study suggests that the
differentially expressed on day 14, suggesting upregulation of miR-21-5p to a lesser extent in L
major molecular rewiring during wound re- vs. NL skin on day 14 results in significantly
epithelialization. We then found that miR-21-5p higher levels of PDCD4 and Maspin, suppressing
was upregulated to a much lesser extent in L day keratinocyte proliferation and migration in
14 as compared to NL day 14, despite its lesional skin and thus delaying wound re-
comparable expression on day 1 (basal level). epithelialization. This study reports the
Overexpression of miR-21-5p (mimic) in comprehensive miRnome of NL and L skin
keratinocyte cell line led to a significant increase during the process of wound healing in
in the expression of proliferation markers (Ki67 individuals with vitiligo and identifies a
and MCM6 mRNA, Ki67 positivity), along with an previously unknown role of Maspin in wound re-
increased keratinocyte migration as assessed by epithelialization, in addition to identifying an
a scratch wound assay. Further, we important role of miR-21 and PDCD4 in wound
demonstrated that miR-21-5p mediates its healing in vitiligo skin.
effects by suppressing the expression of two of

Publications

The long noncoding RNA MALAT1 suppresses miR-211 to confer protection from ultraviolet-mediated DNA damage
in vitiligo epidermis by upregulating sirtuin 1. Brahmbhatt HD, Gupta R, Gupta A, Rastogi S, Misri R, Mobeen A,
Ghosh A, Kothari P, Sitaniya S, Scaria V, Singh A. Br J Dermatol. 2021 Jun;184(6):1132-1142.

Integrated analysis of miRNA- mRNA networks reveals a strong anti-skin cancer signature in vitiligo epidermis.
Singh A, Gupta A, Chowdhary M, Brahmbhatt HD. Exp Dermatol. 2021 Sep;30(9):1309-1319.

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