Page 31 - Biennial Report 2018-20 Jun 2021

which gave high % identity value and coincided with the data provided in published literature
were: Mn dependent superoxide dismutase (24.77 kDa) and glycine rich RNA binding protein
(15.85 kDa). Enzyme linked immunosorbent assay (ELISA) was done for seven food allergic
patients’ sera and for controls. Both the extracts showed one positive reaction with patients’
sera.

Protein extract was prepared for control sample, C3 sample and UV treated sample of Shiitake
mushroom using ammonium bicarbonate buffer. Protein concentration was estimated as 32
mg/g, 69 mg/g and 129.45 mg/g for
Control, C3 and UV treated extract,
respectively using BCA assay. SDS PAGE
profile was performed and integrity of
these protein extracts was analyzed.
Similarly, protein extracts were also
prepared for other samples (Nutra-
ready mix and soft rice mix) followed by
protein estimation (BCA assay) and SDS
PAGE profiling. The protein
concentration was estimated as 31.86
mg/g and 27.82 mg/g for Nutra-ready
mix and soft rice mix samples,
respectively. Specific IgE ELISA with
food allergenic patient sera was
performed. This further validated
potential cross reactivity between
mushroom proteins and other
allergens. Considering a 3-fold increase
in absorbance against normal human
sera, the allergenicity of control and C3 mushroom samples were estimated to be 50-60%. In
future, animal models will be used to study the allergenicity of these mushrooms.
Immunomodulation for allergy therapeutics is also being explored by creating fusion of flagellin
with the P10a allergenic protein of cockroach that has been extensively characterized in IGIB
previously.

UNDERSTANDING CAUSES AND MECHANISM OF EFFEROCYTOSIS IN
RESOLUTION OF INFLAMMATION

Atherosclerosis is a chronic inflammatory disease characterized by impairment in inflammation
resolution response. Inflammation resolution is an active process mediated by crosstalk among
multiple cell types. One of the key players in the inflammation resolution response is the process
of efferocytosis, the phagocytic mechanism by which dead cells are cleared from sites of
inflammation. Defect in clearance of apoptotic cells (efferocytosis) leads to advanced
atherosclerosis plaque progression and clinical manifestations including heart attack and stroke.

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