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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/NMsB/27
Effect of Zno nanoparticles synthesized using bacillus thuringiensis on demographic
parameters of Musca domestica

1*
2
1
Ammara Fatima , Hafsa Iqbal , Hafiz Azhar Ali Khan and Sajid Ali 2
1 Department of Agronomy, University of the Punjab, Lahore
Correspondence: ammara.fatima@lcwu.edu.pk
Abstract

Intensive use of broad-spectrum insecticides can have deleterious effects on insects and the
environment. The use of nanoparticles synthesized from microbes has recently gained
importance as a safe alternative to conventional insecticides. Recently, zinc oxide (ZnO)
nanoparticles synthesized using Bacillus thuringiensis have shown insecticidal potential;
however, in addition to its acute toxicity, it is necessary to determine possible sublethal effects
at the organismal level to understand the toxicity of a new insecticide. Bt-derived enzymes such
as nitrate reductase and other biomolecules play a vital role in the reduction of metal ions to
metal nanoparticles. Here, we assessed the acute toxicity and sublethal effects of ZnO
nanoparticles produced in the culture supernatant of B. thuringiensis ser. israelensis (Bti) as a
reducing agent on the biological traits of Musca domestica. Concentration–response larval
bioassays using different concentrations of ZnO-Bti-supernatant nanoparticles revealed LC10,
−1
LC20, LC50 and LC90 values of 4.17, 6.11, 12.73 and 38.90 μg g of larval diet, respectively.
Exposure of M. domestica larvae to two concentrations (LC10 and LC20) resulted in a
lengthened developmental time (egg to adult) and preoviposition period, and reduced
fecundity, survival, longevity and oviposition period. Furthermore, population parameters
including net reproductive rate, mean generation time, age-specific survival rate, fecundity, life
expectancy and reproductive values, analyzed following age-stage and two-sex life table
theory, were significantly decreased after exposure to these concentrations of ZnO-Bti-
supernatant nanoparticles compared with the control. ZnO-Bti-supernatant nanoparticles were
shown to be toxic to M. domestica. Exposure of M. domestica to low concentrations of ZnO-
Bti-supernatant nanoparticles resulted in negative transgenerational effects on progeny
production in this fly.
Keywords: insecticides, Bacillus thuringiensis.

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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