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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/SAgri/31
Influence of arbuscular mycorrhizal fungi on salinity tolerance of canola

1*
1
Khansa khushi , Saeed Ahmed Qaisrani
1 Department of Environmental Sciences, COMSATS University Islamabad, Vehari- Campus,
61100 Pakistan

Correspondence: Saeed.qaisrani@cuivehari.edu.pk
Abstract

In present study, arbuscular mycorrhizal fungi (AMF) i.e., Glomus mosseae (GM) and Glomus
fasciculatum (GF) amended with Sichar (silicon + biochar) used in the current study. The
salinity treatments were 0 (S0, control) and 120 mM (S1). We were used in the study and
applied at the rate of 50g inoculum per treatment (containing AMF spores, hyphae, root
segments; spore number 25-30 per gram). The treatments were Ct, S0GM, S0GF, S1GM,
S1GF, S0GM+SBC, S0GF+SBC, S1GM+SBC, S1GF+SBC. The treatments were arranged
using the completely randomized design (CRD) and repeated thrice. The crop was harvested
when the 50% pod color were turned pale yellow. The full dose of nitrogen and potash
fertilizers while the half dose of P was applied at the time of harvesting while the remaining
half dose of P was applied at the time of flowering. Ten seeds of canola cultivar CV. Hyola -
401 were sown and then thinned to three. Four irrigations were applied during the whole study.
The AMF along and in combination with SBC improved the growth and development of canola
under salinity stress. Both AMF i.e., GM and GF successfully increased the colonization and
number of spores under salinity but the effect of FM+SBC were more promising. The clear
effect of FM+SBC on the canola plants was noted as the total phenols, total flavonoids and
DPPH contents were decreased under salinity stress. The osmolytes contents were also
reduced. The oil contents in the seeds were also increased in the GF +SBC treatments.
Combination of FM+SBC enhanced macronutrients (N, P, K and Ca) and micronutrients
concentration (Mg, Mn, Cu, and Zn) in plant root and shoot. Hence, overall, the study results
noted that AMF and Sichar biochar application might increase the availability of nutrients
especially NPK for plants. However, the response of both biochar and AMF inoculants may
varied in different soils which needs in depth investigations especially its residual effects at
field levels in different climatic conditions before final recommendations.
Keywords: Biochar; oil contents; Salinity; Canola; Fungi

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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