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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/ETERM/46
Isolation, characterization and identification of dyes degrading bacteria from textile
effluent and textile effluent contaminated soil

Muhammad Usman1, Muhammad Tahir*1, Muhammad Akram1, Abubakar Umer Farooq1,
Tahir Naqqash2, Muhammad Shahid3, Iftikhar Ahmad1, Muhammad Arshad4
1Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus
2Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan
3Department of Bioinformatics and Biotechnology, Govt College University Faisalabad
4Department of Biotechnology, University of Okara

Correspondence: muhammad_tahir@cuivehari.edu.pk
Abstract

All over the world, textile and dyeing industries produce greater quantity of pollutants. Some
dyes don't ever degrade in water. Adsorption and biodegradation are two major ways for
bacterial decolorization of dyes. The objective of the study was to isolate the bacteria inhabiting
dye degrading ability from industrial wastewater. The bacteria were isolated from industrial
wastewater and wastewater contaminated soil by serial dilution method. The bacterial colonies
were purified through streak plate method and stored for further processing. All the bacterial
isolates were tested for enzymatic activity (esterase, cellulase, laccase, protease and chitinase)
and plant growth promoting traits like indole-3-acetic acid (IAA) and phosphate solubilization.
A total 28 pure colonies of bacteria were obtained from the contaminated soil and industrial
wastewater. Among these, 03 bacterial isolates were from dyes wastewater while 25 were from
the soil samples. From the tested isolates, 11 isolates showed esterase activity with variable
strength, 14 isolates showed protease activity, 21 were positive for chitinase activity, 17 for
azo reductase activity, 21 for laccase activity and 15 were positive for cellulase activity. The
isolates obtained from soil and wastewater were tested for IAA and phosphate solubilization.
All the isolates produced IAA ranging from 8-180 µgmL-1 and solubilized phosphate ranging
from 128-226 µgmL-1. A lab experiment was conducted to determine the dyes (Brilliant green
and Congo red) degradation efficiency of the selected isolates either alone or in combination
with Copper Oxide nanoparticles at pH 5 and 7. Results showed that after 15 days, efficiency
of the isolates IWW1-4d and IWS2-3b to degrade brilliant green at pH 5 was 13.3 and 27.9 %,
respectively while at pH 7 the brilliant green degradation efficiency of the isolates IWW1-4d
and IWS2-3b was 62.5 and 74.5%, respectively. Congo red degradation efficiency of the
isolates IWW1-4d and IWS2-3b at pH 5 was 73.3 and 81.7%, respectively while at pH 7
degradation efficiency was 56.7 and 73.3%, respectively. The bacterial isolates IWW1-4d and
IWS2-3b degraded the dyes more efficiently as compared to control and nanoparticles.

Keywords: Nanoparticles, Degradation, chitinase activity, chitinase activity

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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