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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/FWSH/10
Effect of probiotics supplemented rapeseed meal-based diet on performance of Catla
catla fingerlings and lower water pollution

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1*
2
Muhammad Mudassar Shahzad , Syed Makhdoom Hussain , Majid Hussain , Nisar Ahmad ,
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Naveed Ahmad , Laraib Tahir , Fatima Khalid , Kalsoom Akhtar and Sana Bashir
1 Department of Zoology, Division of Science and Technology, University of Education,
Township, Lahore, Pakistan
2 Department of Zoology, Government College University, Lahore, Pakistan
3 Department of Zoology, University of Okara, Okara, Pakistan
4 Department of Zoology, University of Jhang, Jhang, Pakistan
5 Department of Zoology, University of Education, Vehari Campus, Vehari, Pakistan
Correspondence: drmudassarshahzad@gmail.com.
Abstract
A study was conducted to investigate the effects of probiotics supplemented Rapeseed meal-
based diet on growth performance, digestibility of nutrients and absorption of minerals in Catla
catla fingerlings. Fish gut microbiota controls the growth and feed utilization that are highly
influenced by biotic and abiotic factors. Therefore, probiotics were added in rapeseed meal-
based diet for proper digestion and absorption in fish and ultimately higher growth with lower
aquatic pollution. Six test diets were prepared by using different levels of multi-strain
probiotics i.e., 0, 1, 2, 3, 4 and 5 g/Kg (0.0-0.5%) in rapeseed meal-based diet. At the rate of
4% of live wet weight, Catla fingerlings were fed two times a day and faeces samples from
each tank were collected during 70 days of trial period. Growth parameters were analyzed on
fortnightly basis and after trial period chemical analysis of feed and faeces was performed.
According to the results, it was observed that probiotics supplementation (@2 g/kg) in rapeseed
meal based diet resulted in improvement in nutrient digestibility (CP; 72%, fat; 75% and GE;
70%), minerals absorption (Ca; 72%, Na;76%, K; 70% and P; 70%), specific growth rate (SGR;
1.55), improved feed conversion ratio (FCR; 1.22), weight gain (WG; 20.38g) and weight gain
percentage (WG%; 303%) of Catla fingerlings with lower water pollution. It was also noticed
that probiotics supplementation decreased the discharge of minerals and nutrient through faeces
as compared to control diet hence play significant role in reducing water pollution. On the basis
of these results, it was concluded that probiotics inclusion at level of 2 g/kg was useful to
formulate the cost effective and ecofriendly fish feed with the maximum improvement in
growth and fish health by using rapeseed meal-based diet as compared to control and other test
diets.

Keywords: Rapeseed Meal; probiotics; C. catla; growth performance; nutrients digestibility;
minerals absorption.

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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