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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/NMsB/03
Electrochemical investigation of ofloxacin using functionalized multi-walled carbon
nanotubes (mwcnts) paste electrode

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Asma Gul , Muhammad Iqbal Zaman , Abdul Niaz , Muhammad Ibrahim Khan
1 Department of Chemistry, University of Science and technology Bannu-28100, KP Pakistan
Correspondence: iqbalzmn@gmail.com
Abstract

In the present article, we report a novel voltammetric probe for the detection of ofloxacin,
which is based on the charge transfer mechanism between ofloxacin moiety and paste electrode
composed of fictionalized MWCNTs. Ofloxacin is a member of the Fluoroquinolone a class
of synthetic antibiotic, which show a broad spectrum activity against both gram-positive and
gram-negative bacteria, by inactivation of enzymes topoisomerases. Ofloxacin can be
administered both orally and intravenously, where it metabolized to a little extent and within a
duration of 48 hours, a large amount of it excreted as unchanged in urine. Therefore, it is
important to control the content of ofloxacin in pharmaceuticals and biological samples,
determine its residues in foods, and to monitor its concentration in the environment. Thus, a
novel, simple, selective and sensitive voltammetric sensor was designed for detection and
determination of ofloxacin in pharmaceutical formulation and real sample. The functionalized
multiwall carbon nanotubes paste electrode (MWCNTPE) was used for the oxidation of
ofloxacin and determined by using two techniques i.e; the cyclic voltammetry (CV) and
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differential pulse voltammetry (DPV) with the scan rate 0.1Vs and pulse period of 0.1s
respectively in 0.1M Phosphate buffer (electrolyte pH 5) for accumulation time of 60 min. The
ofloxacin was irreversibly adsorbed on the surface of electrode giving an oxidation peak current
at +0.85 V in anodic direction. The designed sensor proved to be very sensitive towards
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ofloxacin with limit of detection being 1×10 M. Similarly, the applied method proved to be
very selective even in the presence of various interfering ions. The low-cost, self-prepared and
easily renewable sensor was successfully applicable for the determination of ofloxacin in
pharmaceutical tablet as well as in biological sample with good percent recovery. It clearly
demonstrating that applied method and proposed sensor are extremely accurate, precise and
suitable for the ofloxacin determination.
Keywords: Carbon nanotubes; Cyclic voltammetry (CV); differential pulse voltammetry
(DPV)

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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