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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/NMsB/32
Use of immobilized biogenic zinc oxide nanoparticles synthesized from Bacillus
marisflavi TEZ7 for methylene blue degradation and textile effluent treatment

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Ali Abbas , Ayesha Iftikhar Cheema , Muhammad Shahid
1 Department of Bioinformatics and Biotechnology, Government College University,
Faisalabad 38000, Pakistan
Correspondence: mshahid@gcuf.edu.pk

Abstract
Industrial wastewater especially from textile industries carries large amount of unwanted
chemical and hazardous/carcinogenic dyes affecting the nutritional, physico-chemical and
biological properties of agricultural lands due to directly released without any proper treatment.
Recently, zinc oxide nanoparticles (ZnO NPs) have received much consideration for the
photocatalytic degradation of dyes and treatment of water pollutants due to their distinctive
physico-chemical and environment friendly properties. In current study, the biosynthesis of
ZnO NPs from Bacillus marisflavi TEZ7, immobilization on calcium alginate (Ca-Alg) beads
and their photocatalytic potential for the degradation of methylene blue (MB) and treatment of
industrial wastewater samples has been investigated. Various material characterization
techniques like X- rays analysis, scanning electron microscopy (SEM), transmission electron
microscopy (TEM), Fourier transformed imaging resonance (FTIR) revealed the biosynthesis
of stable spherical shape ZnO NPs with size ranging from 10.29-26.89 nm. FTIR spectral
analysis indicated the existence of coating protein required for the nanoparticles stabilization.
Biogenic ZnO NPs at varying concentrations (0, 1.0, 1.5 and 2.0 mg/ml water) showed
significant photocatalytic ability at different concentrations (50, 75 and 100 mg/l water) of MB
dye. MB dye degradation analysis by immobilized ZnO NPs at maximum 2 mg/ml
concentration of NPs showed the decolorization difference at different dye concentration 50,
75 and 100 mg/l of MB up to 88.3%, 86.01% and 82.02% respectively under 5 h solar
irradiation. The samples of textile effluents subjected to treatment using biogenic ZnO NPs
exhibited notable reduction in electrical conductivity (EC), pH, total suspended solids (TSS),
turbidity, chemical oxygen demand (COD) and hardness in contrast to non-treated samples
after 5 h solar exposure. Thus, the significant decolorization of MB dye and industrial effluent
treatment using immobilized biogenic ZnO NPs is expected to be cost efficient and more
ecofriendly process in wide range industrial wastewater treatment.

Keywords: Biogenic nanoparticles, Immobilization, Nano-biotechnology Methylene blue,
Photocatalysis, Wastewater, Zinc oxide nanoparticles.

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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