Page 30 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

Contents

S Su ub bj je ec ct t P Pa ag ge e

S Sa af fe et ty y a an nd d L La ab bo or ra at to or ry y R Ru ul le es s 3

C Ch ha ap pt te er r I I: : S Sp pe ec ct tr ro os sc co op py y 7

I- Colorimetry (Visible Spectrophotometry) ………………. 9

I-Colorimetric determination of KMnO4 …………………. 17
II-Colorimetric determination of K2CrO4 ………………... 20

III-Colorimetric determination of FeCl3 …………………. 23

IV- Colorimetric determination of CuSO4 ………………. 26

II- Spectrofluorimetry………………………………………... 29
I-Spectrofluorimetric determination of Quinine Sulphate.. 35

II-Spectrofluorimetric determination of oxybutynin HCl…. 38

C Ch ha ap pt te er r I II I: : C Ch hr ro om ma at to og gr ra ap ph hy y 40

I-Planar chromatography……………………………………. 42

A) Paper chromatography ………………………………… 43

B) Thin-layer chromatography …………………………….. 46

II- Column chromatography…………………………………. 50
Interpretation of chromatograms………………………..…… 54

References …………………………………………………….. 60

2 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5