Page 208 - Applied Pharmacognosy
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Types of Chemical markers
environment and express the desired target product, which
will be secreted from the cell
into the surrounding cell culture
medium
Manufacturing of
biopharmaceuticals
2. down streaming
•When the culture has reached a suitable
end-point, the crude product will be
separated from the host cells in a series
of operations such as centrifugation,
microfiltration and depth filtration. •The
product at this stage will be contained in
