Page 54 - Clinical Biochemistry
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Viral load: Figure 24: HCV Conc.
RealTime PCR:
Sensitive as it can detect
LOD 5-15 IU/mL
Med Microbiol Immunol. 2015; 204(4): 515–525
Therapeutic monitoring:
Important for Peg/IFN and Ribavirin:
Baseline and RGT
DAA monitoring?
• Baseline levels don’t affect duration of therapy
• Viral kinetics during therapy don’t necessarily predict outcome
HCV drug resistance testing:
Genotype specific PCR and Sanger sequencing, online analysis
Figure 25: specific PCR and Sanger sequencing
T54 R155 D168
V36 V55 V170
F43 Y56 Q80 S122 I132 A156 N174
NS3/4A M28 P58 Y93 A553
NS5A Q30 G554
L31 S556
G557
L159 C316 A395 N444 P495 G558
S282 V321 N411 C445 D559
C289 L326 S368 M414 Y448 Y561 S565 I585
C451
Lontok et al., 2015
NS5B
154
