Page 47 - Applied Pharmacognosy
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Classification of Chromatographic Techniques

According to Relative Polarity of Two Phases

❑ Normal-Phase Chromatography               Solutes of Low or
                                            Medium Polarity
   ✓ Stationary phase: Highly polar.
   ✓ Mobile phase: Relatively non-polar.

❑ Reversed-Phase Chromatography             Solutes of High
                                                Polarity
   ✓ Stationary phase: Non-polar.
   ✓ Mobile phase: Polar e.g. acetonitrile

      or methanol.

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