Page 63 - Pharmaceutical Analytical Chemistry III
P. 63
How to draw Calibration (regression)
graphs in instrumental analysis
• The analyst takes a series of different
concentrations of the Analyte (x).
• These standards are measured in the analytical
instrument (y)
• Once the calibration graph has been established, the
unkown concentration of the analyte can be
obtained, by interpolation or using equation.
• This general procedure raises several important
statistical questions:
• 1) Is the calibration graph linear?
• 2) What is the best straight line through these points?
