Page 41 - 01 CARBOHYDRATES for TCM
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Molisch’s Test
.
Based on the dehydration of the carbohydrate by Sulphuric acid to produce an aldehyde, which
condenses with two molecules of α-naphthol, resulting in appearance of a purple ring at the
interface.
A positive test, a soluble carbohydrate will be appear as a purple ring if sulphuric acid is gently
poured in to form a layer below the aqueous solution.