Page 65 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Media for sterility testing

Media for bacteria Media for fungi

1-According to U.S.P: Fluid Sabouraud’s medium
Fluid thioglycolate medium which Temperature = 22 - 25c.

can be used for both aerobic and Incubation period: 10 days.

anaerobic bacteria.

Temperature = 30-32c.

Incubation period: 7days.

2-According to B.P:

Nutrient broth medium for aerobic

bacteria.

Cooked meat medium for

anaerobic bacteria

Controls of sterility testing:

It is made in order to avoid false results, and this includes two main items:

1- Positive control.

2- Negative control.

Positive control: Test the suitability of the medium to allow the growth of

microorganisms or not.

➢ Consists of:

• Two tubes of Fluid thioglycolate:

- One inoculated with aerobic bacteria (ex: E. coli).
- The other one inoculated with anaerobic bacteria (ex: Clostridium

histolyticum).

• Tube of Fluid sabouraud’s medium inoculated with fungi or yeast (ex:

Candida albicans).

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