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General Microbiology & Immunology (PM 401)         Second level                Semester 4                    2024/2025


                    on one or more serum components or whether there is an overabundance of some

                    serum component (thickness of the line).




















                  III. Countercurrent electrophoresis

                   •  In this test the antigen and antibody are placed in wells punched out of an agar

                       gel

                   •   The antigen and antibody are electrophoresed into each other where they form

                       a precipitation line.

                   •   This test only works if conditions can be found where the antigen and antibody

                       have opposite charges.

                   •   This test is primarily qualitative, although from the thickness of the band you

                       can get some measure of quantity.



























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