Page 63 - MNU microbiology theoritical 2025

General Microbiology & Immunology (PM 401) Second level Semester 4 2024/2025

• It contains substances that allow certain groups of bacteria to appear differently

when growing on it (selective agent +differential

agent).

Eg. MacConkey agar

Identification of microorganisms

1. Microscopy

• Staining: Gram, Zeel-Nielsen

• Shape and arrangement

2. Culture

• Media: liquid, solid, differentiate, selective

• Morphology

3. Biochemical

• Oxygen requirement

• Enzymes

• Metabolism-characteristics

4. Serology: antigen antibody interaction

5. Bacteriocin typing

6. Bacteriophage typing

7. Genetic

General Microbiology & Immunology (PM 401) theoretical handbook Page 52