Page 47 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Advantages

• Simple staining is a very simple method to perform, which stains the organism by

using a single reagent.

• It is a rapid method that reduces performance time by taking only 3-5 minutes.

• Simple staining helps to examine or elucidate the bacterial shape, size and
arrangement.

• It also helps us to differentiate the bacterial cells from the non-living structures.
• Simple staining can be useful in the preliminary study of the bacteria’s morphological

characteristics.

Disadvantages

• It does not give much information about the cell apart from the bacteria’s

morphological characteristics.

• Through simple staining, we cannot classify a particular type of organism.

General Microbiology & Immunology (PM 401) Practical note Page 36