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Damage was evaluated with gel electrophoresis [3] and high-performance liquid chromatography. These
results show that the hydrated electrons induce the detachment of cisplatin adducts from the
oligonucleotides. We propose that cisplatin leads to the capture of a hydrated electron by the
oligonucleotide giving rise to a transient anion, which dissociates, breaking of one or two guanine-cisplatin
bonds that is dissociative electron transfer. Furthermore, much higher levels of base damages are
detected when cisplatin adducts are present. This damage was largely localized at cisplatin attachment
sites (guanines) but also on thymines. Finaly, the rate constant for the hydrated electron interactions with
cisplatin-oligonucleotide was measured using pulse radiolysis. The large rate contant (2, 23x10^10 Lmol^-
1s^-1) demonstrates that the presence of a cisplatin adduct leads to a strong interaction with hydrated
electrons. Our results have revealed the probable mechanism by which cisplatin adducts sensitize DNA to
the indirect effects of ionising radiation. [1]Reedijk, J. Platinum Anticancer Coordination Compounds:
Study of DNA Binding Inspires New Drug Design. Eur. Inorg. Chem. 2009, 1303-1312 [2] Zheng, Y.; Hunting,
D. J.; Ayotte, P.; Sanche, L. Role of Secondary Low-Energy Electrons in the Concomitant Chemoradiation
Therapy of Cancer. Phys. Rev. Lett. 2008, 100, 198101/1−198101/4. [3] Behmand, B.; Cloutier, P.;
Girouard, S.; Wagner, J. R.; Sanche, L.; Hunting, D. Hydrated Electrons React with High Specificity with
Cisplatin Bound to Single-Stranded DNA. J. Phys. Chem. B. 2013, 117, 15994-15999.
(PS1-05) Emissions from 900 MHz gsm antennae damage DNA of hibiscus sabdariffa. Olusegun A.
Oluwajobi, M Sc, PhD, and Olamide A. Falusi, PhD, Federal University of Technology, Minna, Minna,
Nigeria
Potted plants of Hibiscus sabdariffa L. were raised at 100, 200, 300 and 400metres from 900MHz
GSM antennae in three selected locations in Minna metropolis of Niger State, Nigeria. Control plants were
raised in Gnague, a settlement without GSM signals, in the outskirt of Minna. Fresh young leaves were
collected from each plant at two, eight and twenty-four weeks after sowing and DNA were extracted from
the leaf samples. RAPD PCR products were run on Agarose gel and data obtained were used to make
factorial analyses. The results showed that DNA of samples from the control and those from 400m of
location C remain unchanged throughout the period of the experiment while the DNA from all other
samples raised under the influence of the emissions from the GSM antenna showed various levels of
changes, estimated at 48.72%. Damage occurred in 58.33% of the samples at 8weeks exposure while
33.33% of the samples were damaged only at 24weeks exposure. 8.33% of the samples did not show DNA
damage. Some 33.33% of the samples showed that DNA damage occurred twice, both at 8 and 24 weeks
of exposure. These results therefore showed that the electromagnetic radiation from 900MHz GSM
antenna, though regarded as very low and weak is potent enough to cause damage to DNA of Hibiscus
sabdariffa plant even within 8 weeks of exposure.
104 | P a g e
results show that the hydrated electrons induce the detachment of cisplatin adducts from the
oligonucleotides. We propose that cisplatin leads to the capture of a hydrated electron by the
oligonucleotide giving rise to a transient anion, which dissociates, breaking of one or two guanine-cisplatin
bonds that is dissociative electron transfer. Furthermore, much higher levels of base damages are
detected when cisplatin adducts are present. This damage was largely localized at cisplatin attachment
sites (guanines) but also on thymines. Finaly, the rate constant for the hydrated electron interactions with
cisplatin-oligonucleotide was measured using pulse radiolysis. The large rate contant (2, 23x10^10 Lmol^-
1s^-1) demonstrates that the presence of a cisplatin adduct leads to a strong interaction with hydrated
electrons. Our results have revealed the probable mechanism by which cisplatin adducts sensitize DNA to
the indirect effects of ionising radiation. [1]Reedijk, J. Platinum Anticancer Coordination Compounds:
Study of DNA Binding Inspires New Drug Design. Eur. Inorg. Chem. 2009, 1303-1312 [2] Zheng, Y.; Hunting,
D. J.; Ayotte, P.; Sanche, L. Role of Secondary Low-Energy Electrons in the Concomitant Chemoradiation
Therapy of Cancer. Phys. Rev. Lett. 2008, 100, 198101/1−198101/4. [3] Behmand, B.; Cloutier, P.;
Girouard, S.; Wagner, J. R.; Sanche, L.; Hunting, D. Hydrated Electrons React with High Specificity with
Cisplatin Bound to Single-Stranded DNA. J. Phys. Chem. B. 2013, 117, 15994-15999.
(PS1-05) Emissions from 900 MHz gsm antennae damage DNA of hibiscus sabdariffa. Olusegun A.
Oluwajobi, M Sc, PhD, and Olamide A. Falusi, PhD, Federal University of Technology, Minna, Minna,
Nigeria
Potted plants of Hibiscus sabdariffa L. were raised at 100, 200, 300 and 400metres from 900MHz
GSM antennae in three selected locations in Minna metropolis of Niger State, Nigeria. Control plants were
raised in Gnague, a settlement without GSM signals, in the outskirt of Minna. Fresh young leaves were
collected from each plant at two, eight and twenty-four weeks after sowing and DNA were extracted from
the leaf samples. RAPD PCR products were run on Agarose gel and data obtained were used to make
factorial analyses. The results showed that DNA of samples from the control and those from 400m of
location C remain unchanged throughout the period of the experiment while the DNA from all other
samples raised under the influence of the emissions from the GSM antenna showed various levels of
changes, estimated at 48.72%. Damage occurred in 58.33% of the samples at 8weeks exposure while
33.33% of the samples were damaged only at 24weeks exposure. 8.33% of the samples did not show DNA
damage. Some 33.33% of the samples showed that DNA damage occurred twice, both at 8 and 24 weeks
of exposure. These results therefore showed that the electromagnetic radiation from 900MHz GSM
antenna, though regarded as very low and weak is potent enough to cause damage to DNA of Hibiscus
sabdariffa plant even within 8 weeks of exposure.
104 | P a g e
