Page 201 - 2014 Printable Abstract Book
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considered when estimating the risk of radiogenic skin cancer and of other epithelial cancers in astronauts
on deep space missions.
(PS3-20) Rapid and easy staining for centromere and telomere analysis for radiation cytogenetics.Ian
M. Cartwright, M.S.; Matthew D. Genet; Takamitsu A. Kato, PhD ;Colorado State University, Ft Collins, CO
Traditional radiation cytogenetics rely on structural and morphological differences of damaged
chromosomes. Fluorescence painting overcomes this problem but there are still problems for time for
staining protocol and special techniques associated with good painting output. We developed two PNA
painting methods especially for centromere and telomere. The first method is microwave mediated rapid
FISH. Slides were microwaved with PNA probes. Denature, hybridization and incubation processes
completes within 3 minutes. Another protocol is slow but more reproducible nontraditional hybridization
assay. Simply PNA probes were incubated with slides without denature for overnight in room
temperature. These two assays provide time and easiness for researchers to carry out radiation induced
chromosome aberration analysis. Fluorescence signals based chromosome aberration scoring is more
accurate and easy for analysis.
(PS3-21) Convection-enhanced delivery of platinum-based drugs: chemotherapy plus time-optimized
radiotherapy in f98 glioma-bearing fischer rats. Minghan Shi; Brigitte Guérin; David Fortin; Benoit
Paquette; and Léon Sanche, Université de Sherbrooke, Sherbrooke, Canada
Glioblastoma is the most common and aggressive primary brain tumor in adults. The prognosis of
patients with glioblastoma remains poor with the current treatments, which include platinum-based
drugs. These latter have been extensively studied as chemotherapeutic agents and radiosensitizers for
decades. In this study, we use intra-tumoral convection-enhanced delivery (CED) to inject different
platinum-based drugs (cisplatin, carboplatin, oxaliplatin, lipoplatinTM, lipoxalTM) directly into the tumor
of F98 glioma bearing rats site and also treat them with gamma rays. The survival time of the rats treated
with different administration routes (CED, intra-arterial (i.a.), and intra-venous (i.v.)) was measured. Our
previous in vitro and in vivo studies showed that a higher amount of DNA-platinum adducts conferred a
more efficient concomitant treatment. Thus, to determine the time of maximum content of DNA-bound
platinum adducts of oxaliplatin and carboplatin in the tumor and normal brain around the tumor, these
tissues were analyzed at 4, 24 and 48 hours after CED by inductively coupled plasma mass spectrometry
(ICP-MS). Survival times of rats treated with carboplatin and oxaliplatin plus radiotherapy were recorded
at 4 and 24 hours after CED. Results: Among the tested drugs, carboplatin offered the best median survival
time (42.5 days). It was further increased by combination with radiotherapy (54.0 days). Comparing to
other routes of administration, CED of platinum-based chemotherapy drugs offered the best or equivalent
survival time. The DNA-bound platinum adducts were highest at 4 hours after CED in either oxaliplatin or
carboplatin group. However, no significant difference in survival time was found in between radiotherapy
at 4 and 24 hours after CED. But more acute toxicity associated with the treatment was observed in
radiotherapy at 4 hours after CED-based chemotherapy. This may due to the high amount of DNA-
platinum adducts in the normal brain tissue around tumor at 4 hours after CED, which created more
damage on the normal brain tissue. Conclusion: Carboplatin injected by CED and followed 24 hours later
by radiotherapy resulted in the best survival in F98 glioma bearing rats.
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on deep space missions.
(PS3-20) Rapid and easy staining for centromere and telomere analysis for radiation cytogenetics.Ian
M. Cartwright, M.S.; Matthew D. Genet; Takamitsu A. Kato, PhD ;Colorado State University, Ft Collins, CO
Traditional radiation cytogenetics rely on structural and morphological differences of damaged
chromosomes. Fluorescence painting overcomes this problem but there are still problems for time for
staining protocol and special techniques associated with good painting output. We developed two PNA
painting methods especially for centromere and telomere. The first method is microwave mediated rapid
FISH. Slides were microwaved with PNA probes. Denature, hybridization and incubation processes
completes within 3 minutes. Another protocol is slow but more reproducible nontraditional hybridization
assay. Simply PNA probes were incubated with slides without denature for overnight in room
temperature. These two assays provide time and easiness for researchers to carry out radiation induced
chromosome aberration analysis. Fluorescence signals based chromosome aberration scoring is more
accurate and easy for analysis.
(PS3-21) Convection-enhanced delivery of platinum-based drugs: chemotherapy plus time-optimized
radiotherapy in f98 glioma-bearing fischer rats. Minghan Shi; Brigitte Guérin; David Fortin; Benoit
Paquette; and Léon Sanche, Université de Sherbrooke, Sherbrooke, Canada
Glioblastoma is the most common and aggressive primary brain tumor in adults. The prognosis of
patients with glioblastoma remains poor with the current treatments, which include platinum-based
drugs. These latter have been extensively studied as chemotherapeutic agents and radiosensitizers for
decades. In this study, we use intra-tumoral convection-enhanced delivery (CED) to inject different
platinum-based drugs (cisplatin, carboplatin, oxaliplatin, lipoplatinTM, lipoxalTM) directly into the tumor
of F98 glioma bearing rats site and also treat them with gamma rays. The survival time of the rats treated
with different administration routes (CED, intra-arterial (i.a.), and intra-venous (i.v.)) was measured. Our
previous in vitro and in vivo studies showed that a higher amount of DNA-platinum adducts conferred a
more efficient concomitant treatment. Thus, to determine the time of maximum content of DNA-bound
platinum adducts of oxaliplatin and carboplatin in the tumor and normal brain around the tumor, these
tissues were analyzed at 4, 24 and 48 hours after CED by inductively coupled plasma mass spectrometry
(ICP-MS). Survival times of rats treated with carboplatin and oxaliplatin plus radiotherapy were recorded
at 4 and 24 hours after CED. Results: Among the tested drugs, carboplatin offered the best median survival
time (42.5 days). It was further increased by combination with radiotherapy (54.0 days). Comparing to
other routes of administration, CED of platinum-based chemotherapy drugs offered the best or equivalent
survival time. The DNA-bound platinum adducts were highest at 4 hours after CED in either oxaliplatin or
carboplatin group. However, no significant difference in survival time was found in between radiotherapy
at 4 and 24 hours after CED. But more acute toxicity associated with the treatment was observed in
radiotherapy at 4 hours after CED-based chemotherapy. This may due to the high amount of DNA-
platinum adducts in the normal brain tissue around tumor at 4 hours after CED, which created more
damage on the normal brain tissue. Conclusion: Carboplatin injected by CED and followed 24 hours later
by radiotherapy resulted in the best survival in F98 glioma bearing rats.
199 | P a g e
