Page 235 - 2014 Printable Abstract Book
P. 235
PS4 CELL AND TISSUE, STEM CELLS, RADIATION MICROENVIRONMENT, EXPERIMENTAL
THERAPEUTICS, RADIATION RESPONSE CNS, TRANSLATIONAL RESEARCH
(PS4-01)Implications of low-dose x-ray and heavy ion exposure on wound healing pathways in a 3D skin
1
1
1
2;3
tissue model. Jake Pirkkanen ; Claere von Neubeck ; Daniel J. Mullen ; Catherine L. Winter ; and
1
Marianne B. Sowa, Pacific Northwest National Laboratory, Richland, WA ; German Cancer Consortium
1
(DKTK) OncoRay - National Center for Radiation Research in Oncology Medical Faculty and University
2
Hospital Carl Gustav Carus Technische Universität Dresden, Dresden, Germany ; and German Cancer
Research Center (DKFZ), Heidelberg, Germany
3
There is a growing concern over the potential risks created by exposure to radiation in our
everyday lives, especially because of its potential as a carcinogen. We are exposed to low doses of ionizing
radiation from a number of sources outside of the natural background, such as medical diagnostic imaging,
security screenings, commercial airline and space flight, and perhaps most notably, from nuclear incidents
such as the Fukushima disaster. However, there is considerable uncertainty in the epidemiological data
for low-dose radiation exposure (<100 cGy) and presently, appropriate predictions are challenging to
make. This necessitates a relevant experimental system with which we can study cellular, molecular and
tissue level processes. To this end, we investigated the effects of low-dose ionizing radiation on wound
healing responses in a 3D human skin tissue model. The wound healing pathway serves as an ideal model
for examining the effects of low-dose radiation because it is a comprehensive procedure involving a
number of common cellular processes and because faults in the wound healing pathway have been
implicated as potential causes for cancer. We exposed in vitro 3D human skin cell tissues to 3, 10, and
200 cGy of X-rays, as well as varying fluences of heavy ion radiation. At 3, 24, and 72 hours post-irradiation,
quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blot analysis,
immunostaining, and cell proliferation assays were performed to assess the effect of low-dose radiation
on the wound healing pathway through differential gene expression and cell growth and survival rates.
Our analyses show even exposure to doses of X-ray radiation as low as 10 cGy, the equivalent of a CT scan,
can induce up to a nine-fold change in the expression of important wound healing genes such as EGR1,
CTGF, FN1, CYR61, FGF7, TIMP1, SERPINE1, and TGF-β1, and can affect the cell count and rate of
proliferation in both the dermal and epidermal layers of our tissue samples. We also found that the cells’
response to low-dose radiation exposure often differed significantly from that of high-dose radiation.
These results further our understanding of the effects of low-dose ionizing radiation exposure on human
health, and the cellular mechanisms that underlie them.
(PS4-02) Non-invasive, in-vivo assessment of radiation induced effects on solid GOT1 tumors using
2
1
diffusion weighted magnetic resonance imaging. Mikael Montelius, MSc ; Maria Ljungberg ; and Eva
Forsell-Aronsson, 1;2 Dept. of Radiation Physics, Inst. of Clinical science, Sahlgrenska university hospital,
1
Gothenburg, Sweden and Sahlgrenska University Hospital, Division of Medical Physics and Medical
Engineering, Gothenburg, Sweden
2
Primarily, radiation therapy (RT) aims to induce tumor cell apoptosis/necrosis, but effects on the
1
tumor capillary endothelium and microenvironment could affect RT outcome significantly . Therefore, RT
response assessments that include perfusion would be of interest. Diffusion weighted MRI (DWI) is
233 | P a g e
THERAPEUTICS, RADIATION RESPONSE CNS, TRANSLATIONAL RESEARCH
(PS4-01)Implications of low-dose x-ray and heavy ion exposure on wound healing pathways in a 3D skin
1
1
1
2;3
tissue model. Jake Pirkkanen ; Claere von Neubeck ; Daniel J. Mullen ; Catherine L. Winter ; and
1
Marianne B. Sowa, Pacific Northwest National Laboratory, Richland, WA ; German Cancer Consortium
1
(DKTK) OncoRay - National Center for Radiation Research in Oncology Medical Faculty and University
2
Hospital Carl Gustav Carus Technische Universität Dresden, Dresden, Germany ; and German Cancer
Research Center (DKFZ), Heidelberg, Germany
3
There is a growing concern over the potential risks created by exposure to radiation in our
everyday lives, especially because of its potential as a carcinogen. We are exposed to low doses of ionizing
radiation from a number of sources outside of the natural background, such as medical diagnostic imaging,
security screenings, commercial airline and space flight, and perhaps most notably, from nuclear incidents
such as the Fukushima disaster. However, there is considerable uncertainty in the epidemiological data
for low-dose radiation exposure (<100 cGy) and presently, appropriate predictions are challenging to
make. This necessitates a relevant experimental system with which we can study cellular, molecular and
tissue level processes. To this end, we investigated the effects of low-dose ionizing radiation on wound
healing responses in a 3D human skin tissue model. The wound healing pathway serves as an ideal model
for examining the effects of low-dose radiation because it is a comprehensive procedure involving a
number of common cellular processes and because faults in the wound healing pathway have been
implicated as potential causes for cancer. We exposed in vitro 3D human skin cell tissues to 3, 10, and
200 cGy of X-rays, as well as varying fluences of heavy ion radiation. At 3, 24, and 72 hours post-irradiation,
quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blot analysis,
immunostaining, and cell proliferation assays were performed to assess the effect of low-dose radiation
on the wound healing pathway through differential gene expression and cell growth and survival rates.
Our analyses show even exposure to doses of X-ray radiation as low as 10 cGy, the equivalent of a CT scan,
can induce up to a nine-fold change in the expression of important wound healing genes such as EGR1,
CTGF, FN1, CYR61, FGF7, TIMP1, SERPINE1, and TGF-β1, and can affect the cell count and rate of
proliferation in both the dermal and epidermal layers of our tissue samples. We also found that the cells’
response to low-dose radiation exposure often differed significantly from that of high-dose radiation.
These results further our understanding of the effects of low-dose ionizing radiation exposure on human
health, and the cellular mechanisms that underlie them.
(PS4-02) Non-invasive, in-vivo assessment of radiation induced effects on solid GOT1 tumors using
2
1
diffusion weighted magnetic resonance imaging. Mikael Montelius, MSc ; Maria Ljungberg ; and Eva
Forsell-Aronsson, 1;2 Dept. of Radiation Physics, Inst. of Clinical science, Sahlgrenska university hospital,
1
Gothenburg, Sweden and Sahlgrenska University Hospital, Division of Medical Physics and Medical
Engineering, Gothenburg, Sweden
2
Primarily, radiation therapy (RT) aims to induce tumor cell apoptosis/necrosis, but effects on the
1
tumor capillary endothelium and microenvironment could affect RT outcome significantly . Therefore, RT
response assessments that include perfusion would be of interest. Diffusion weighted MRI (DWI) is
233 | P a g e
