Page 266 - 2014 Printable Abstract Book
P. 266
1
(PS4-51) Stem cells therapy for cutaneous radiation lesion: from bench to bedside. Radia TAMARAT ;
2
3
1
1
Jean Jacques Lataillade ; Eric Bey ; Patrick Gourmelon ; and Marc Benderitter, IRSN, Fontenay-aux-Roses,
1
2
France ; Centre de Transfusion des A rmées, Clamart, France ; and Hopital d'Instruction des Armées Percy,
Percy, France
3
The medical management of severe radiation burns after accidental overexposure to ionizing
radiation was a major therapeutic challenge unresolved since these 5 last years with the classical
therapeutic approach derived from the management of thermal or electrical burns. The evolution of the
radiation lesion often becomes uncontrolled and the final option is a last surgical act leading to a high
morbidity and disability. Thus, adult stem cell therapy was postulated to favour also the radiation burn
healing process. Preclinical benefit of MSC injection for ulcerated skin and muscle restoration after high
dose radiation exposure has been successfully demonstrated in our group. The clinical benefit of MSC
combined or not to surgery in promoting the healing of cutaneaous radiation syndrome (CRS) was
successfully demonstrated in seven patients overexposed accidentally to radiation since 2006. Overall,
the current data indicate that although MSC were first propose for therapeutic purposes in regenerative
medicine on the basis of their stem cell like qualities and from other characteristics such as their anti
proliferative and anti inflammatory properties. Ultimately, in the recent years, in our group we suggest
others progenitors cells (as Adipose-derived stem cells or Endothelial progenitor cells or Co
transplantation of different type of stem cells) to be proposed as new therapeutic strategy to potentialize
beneficial effect in skin reconstitution. Therefore, the development of new therapeutic approach using
adult stem cells as a potential to control inflammation, accelerate reepithelialisation and vessel growth
constitute a major challenge for tissue regeneration or repair after irradiation.
(PS4-52) Tumor Radioresistance: Contribution of tumor cells, tumor endothelial cells, and cancer stem
cells in 3D multicell cocultures. Ryan Chan; Pallavi Sethi; Amar Jyoti; Meenakshi Upreti; Ronald McGarry;
and William St. Clair, University of Kentucky, Lexington, KY
Lung cancer is by far the most common cause of worldwide cancer deaths and is the second most
common cancer diagnosed in men and women in the United States. Accounting for 90% of all lung cancer
cases in the United States, patients with advanced non-small cell lung cancer (NSCLC) have few effective
treatment options. Radiation therapy has been regarded as the main treatment strategy for NSCLC
patients historically. However, radioresistance of the cancer cells is a key issue limiting the efficacy of
radiation therapy. Cancer cells exhibiting stem cell-like features, known as cancer stem cells (CSCs), exhibit
radioresistance and have been reported to confer their radioresistance to the regrowing tumor. Recent
studies have revealed that the impact of hypoxia and interaction of these CSCs with stromal elements in
the surrounding microenvironment enhances tumor cell survival mechanisms. Due to the innate
radioresistance of CSCs, the ability of the microenvironment (particularly the endothelial cells) to confer
radioresistance to tumor cells, and the interactions observed between endothelial cells and CSCs, it is
imperative to develop a model encompassing the tumor, the tumor microenvironment, and the CSCs
within the microenvironment. To that end we have developed a human 3D co-culture system for non-
small cell lung cancer comprising of tumor cells, endothelial cells and cancer stem cells. This lung cancer
model allows for delineating the contribution of each cell type as they are color coded expressing different
fluorescent proteins. Conventional radiotherapy for NSCLC utilizes a fractionated regimen of 60 Gy given
264 | P a g e
(PS4-51) Stem cells therapy for cutaneous radiation lesion: from bench to bedside. Radia TAMARAT ;
2
3
1
1
Jean Jacques Lataillade ; Eric Bey ; Patrick Gourmelon ; and Marc Benderitter, IRSN, Fontenay-aux-Roses,
1
2
France ; Centre de Transfusion des A rmées, Clamart, France ; and Hopital d'Instruction des Armées Percy,
Percy, France
3
The medical management of severe radiation burns after accidental overexposure to ionizing
radiation was a major therapeutic challenge unresolved since these 5 last years with the classical
therapeutic approach derived from the management of thermal or electrical burns. The evolution of the
radiation lesion often becomes uncontrolled and the final option is a last surgical act leading to a high
morbidity and disability. Thus, adult stem cell therapy was postulated to favour also the radiation burn
healing process. Preclinical benefit of MSC injection for ulcerated skin and muscle restoration after high
dose radiation exposure has been successfully demonstrated in our group. The clinical benefit of MSC
combined or not to surgery in promoting the healing of cutaneaous radiation syndrome (CRS) was
successfully demonstrated in seven patients overexposed accidentally to radiation since 2006. Overall,
the current data indicate that although MSC were first propose for therapeutic purposes in regenerative
medicine on the basis of their stem cell like qualities and from other characteristics such as their anti
proliferative and anti inflammatory properties. Ultimately, in the recent years, in our group we suggest
others progenitors cells (as Adipose-derived stem cells or Endothelial progenitor cells or Co
transplantation of different type of stem cells) to be proposed as new therapeutic strategy to potentialize
beneficial effect in skin reconstitution. Therefore, the development of new therapeutic approach using
adult stem cells as a potential to control inflammation, accelerate reepithelialisation and vessel growth
constitute a major challenge for tissue regeneration or repair after irradiation.
(PS4-52) Tumor Radioresistance: Contribution of tumor cells, tumor endothelial cells, and cancer stem
cells in 3D multicell cocultures. Ryan Chan; Pallavi Sethi; Amar Jyoti; Meenakshi Upreti; Ronald McGarry;
and William St. Clair, University of Kentucky, Lexington, KY
Lung cancer is by far the most common cause of worldwide cancer deaths and is the second most
common cancer diagnosed in men and women in the United States. Accounting for 90% of all lung cancer
cases in the United States, patients with advanced non-small cell lung cancer (NSCLC) have few effective
treatment options. Radiation therapy has been regarded as the main treatment strategy for NSCLC
patients historically. However, radioresistance of the cancer cells is a key issue limiting the efficacy of
radiation therapy. Cancer cells exhibiting stem cell-like features, known as cancer stem cells (CSCs), exhibit
radioresistance and have been reported to confer their radioresistance to the regrowing tumor. Recent
studies have revealed that the impact of hypoxia and interaction of these CSCs with stromal elements in
the surrounding microenvironment enhances tumor cell survival mechanisms. Due to the innate
radioresistance of CSCs, the ability of the microenvironment (particularly the endothelial cells) to confer
radioresistance to tumor cells, and the interactions observed between endothelial cells and CSCs, it is
imperative to develop a model encompassing the tumor, the tumor microenvironment, and the CSCs
within the microenvironment. To that end we have developed a human 3D co-culture system for non-
small cell lung cancer comprising of tumor cells, endothelial cells and cancer stem cells. This lung cancer
model allows for delineating the contribution of each cell type as they are color coded expressing different
fluorescent proteins. Conventional radiotherapy for NSCLC utilizes a fractionated regimen of 60 Gy given
264 | P a g e
