Page 286 - 2014 Printable Abstract Book
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radiosensitizing event in 6 NSCLC cell lines and in a syngeneic tumor model. Use of the NPM1 null and WT
MEFs allowed us to demonstrate that YTR107-mediated radiosensitization requires NPM1. We also found
that YTR107 is well tolerated in mice, does not produce overt toxicity, and does not potentiate radiation-
mediated pulmonary fibrosis. These data suggest that NPM1 represents a potential molecular target for
radiosensitization of NSCLC. Supported in part by RO1CA140409, Vanderbilt-Ingram Cancer CTR Grant P30
CA68485 and the Vanderbilt SPORE in lung cancer P50CA090949.
(PS4-89) Combined effect of gold nanoparticles, platinum compounds, radiation and hyperthermia in
treatment of cancer. Gabriel Charest, PhD and Léon Sanche, PhD, Université de Sherbrooke, Sherbrooke,
Canada
It is well accepted that fighting cancer requires multimodal therapy. Ideally, these therapies
should bring to an additive or a synergistic effect. In earlier publications we have demonstrated that
carboplatin is a good radiosensitizer and gold nanoparticles acts as a radio-enhancer by capturing the
energy of ionizing radiation and by redistributing this energy locally as damaging low energy electrons. In
this study, we have treated two cell lines (F98: glioblastoma, HCT116: colorectal cancer) with a
combination of three treatment modalities (chemotherapy, radiotherapy and hyperthermia). The aim was
to increase the radiation effect with a radiosensitizer/ radio-enhancer (liposomal platinum compounds
and gold nanoparticles) and hyperthermia. In vitro clonogenic assays with increasing dose of drug and/or
radiation were performed with or without hyperthermia and the dose response curves were compared.
The in vivo experiments with colorectal cancer cell line HCT116 implanted in Nu/Nu nude mice were
performed under similar conditions. The combination of carboplatin, gold nanoparticles and radiation
results in a synergistic effect enhancing cells damage, which led to better tumor control. Moreover, the
liposomal formulation is known to accumulate preferentially into the tumor compartment by the EPR
effect, which increases the chemo-radiotherapy action preferentially to the tumor area.
284 | P a g e
MEFs allowed us to demonstrate that YTR107-mediated radiosensitization requires NPM1. We also found
that YTR107 is well tolerated in mice, does not produce overt toxicity, and does not potentiate radiation-
mediated pulmonary fibrosis. These data suggest that NPM1 represents a potential molecular target for
radiosensitization of NSCLC. Supported in part by RO1CA140409, Vanderbilt-Ingram Cancer CTR Grant P30
CA68485 and the Vanderbilt SPORE in lung cancer P50CA090949.
(PS4-89) Combined effect of gold nanoparticles, platinum compounds, radiation and hyperthermia in
treatment of cancer. Gabriel Charest, PhD and Léon Sanche, PhD, Université de Sherbrooke, Sherbrooke,
Canada
It is well accepted that fighting cancer requires multimodal therapy. Ideally, these therapies
should bring to an additive or a synergistic effect. In earlier publications we have demonstrated that
carboplatin is a good radiosensitizer and gold nanoparticles acts as a radio-enhancer by capturing the
energy of ionizing radiation and by redistributing this energy locally as damaging low energy electrons. In
this study, we have treated two cell lines (F98: glioblastoma, HCT116: colorectal cancer) with a
combination of three treatment modalities (chemotherapy, radiotherapy and hyperthermia). The aim was
to increase the radiation effect with a radiosensitizer/ radio-enhancer (liposomal platinum compounds
and gold nanoparticles) and hyperthermia. In vitro clonogenic assays with increasing dose of drug and/or
radiation were performed with or without hyperthermia and the dose response curves were compared.
The in vivo experiments with colorectal cancer cell line HCT116 implanted in Nu/Nu nude mice were
performed under similar conditions. The combination of carboplatin, gold nanoparticles and radiation
results in a synergistic effect enhancing cells damage, which led to better tumor control. Moreover, the
liposomal formulation is known to accumulate preferentially into the tumor compartment by the EPR
effect, which increases the chemo-radiotherapy action preferentially to the tumor area.
284 | P a g e
