Page 305 - 2014 Printable Abstract Book
P. 305
reprogramming events from occurring, with the long-term goal of identifying opportunities to significantly
improve the benefit from radiation therapy for GBM patients.
(PS5-31) Low-dose ionizing radiation inhibits IgE-mediated allergic reaction. Ji Young Kim; Hae Mi Joo;
Su Jin Kang; Seon Young Nam; Cha Soon Kim; and Kwang Hee Yang, Radiation Research Health Institute,
Seoul, Korea, Republic of
Ionizing radiation can have different biological effects according to dose and dose rate. Especially,
the biological effect of low dose radiation is still a matter of debate. Low-dose whole body gamma
irradiation has been shown to activate immune responses in several ways, however, the effect and
mechanism of irradiation on allergic reaction remains poorly understood. Earlier, we reported that low-
dose ionizing radiation inhibited mediator release in FcεRI-mediated RBL-2H3 mast cell activation. In this
study, we investigated whether low-dose radiation inhibit mediator release in human mast cells (HMC-
1(5C6) and LAD2) or allergic reaction in passive cutaneous anaphylaxis and late-phase cutaneous mouse
model. High dose radiation induced cell death but, low dose ionizing radiation of < 0.5 Gy did not induced
mast cell death. Low-dose ionizing radiation that did not induce cell death significantly suppressed
histamine release from human mast cells (HMC-1(5C6) and LAD2) activated by antigen-antibody reaction.
In order to reveal the inhibitory mechanism of mediator released by low dose ionizing radiation, we
examined the phosphorylation of intracellular signaling molecules such as Lyn, Syk, PLCγ, PKCs, and
2+
2+
2+
intracellular free Ca concentration ([Ca ]i). The phosphorylation of signaling molecules and [Ca ]i via
stimulation of FcεRI receptor was specifically inhibited by low dose ionizing radiation. In agreement with
its in vitro effect, ionizing radiation also significantly inhibited passive cutaneous anaphylaxis (PCA)
reaction and late-phase cutaneous response in anti-dinitrophenyl (DNP) IgE-sensitized mice. These results
indicate that ionizing radiation inhibits both mast cell-mediated immediate and delayed-type allergic
reactions in vivo and in vitro.
(PS5-32) A spreadsheet for detection of possible data fabrication in numerical data sets of the type
1
frequently encountered in cell and radiation biology survival studies. Helene Z. Hill, PhD and Joel H. Pitt,
1
2
2
PhD ; Rutgers NJ Medical School, Newark, NJ and Renaissance Associates, Princeton, NJ
The growing awareness of the frequency of falsified and fabricated data in scientific and medical
reports highlights the need for tools to identify problematic data prior to publication. Programs to detect
plagiarism are routinely used by a number of publishers and have led to a decrease in this type of
misconduct. Image manipulation can be detected by using programs such as one available on the Office
of Research Integrity website. Routine use of such programs will help to curtail this type of fraud, as well.
We have constructed a spreadsheet that can process numerical data sets of a type frequently encountered
in cell and radiation biology survival studies to detect possible data fabrication. Such detection is based
on the fact that fabrication may be indicated by the presence of unusual, unexpected patterns in data.
For example, rightmost terminal digits of counts taken from particle and scintillation counters should be
relatively uniform, whereas numbers invented by individuals do not generally have uniform terminal
digits. Hence, significant deviation from uniformity of the terminal digits of count data could be cause for
concern. Another suspect pattern, more specific to survival studies in radiation biology and pharmacology,
where samples are generally tested in triplicate, is an unusually high frequency of triples that include
303 | P a g e
improve the benefit from radiation therapy for GBM patients.
(PS5-31) Low-dose ionizing radiation inhibits IgE-mediated allergic reaction. Ji Young Kim; Hae Mi Joo;
Su Jin Kang; Seon Young Nam; Cha Soon Kim; and Kwang Hee Yang, Radiation Research Health Institute,
Seoul, Korea, Republic of
Ionizing radiation can have different biological effects according to dose and dose rate. Especially,
the biological effect of low dose radiation is still a matter of debate. Low-dose whole body gamma
irradiation has been shown to activate immune responses in several ways, however, the effect and
mechanism of irradiation on allergic reaction remains poorly understood. Earlier, we reported that low-
dose ionizing radiation inhibited mediator release in FcεRI-mediated RBL-2H3 mast cell activation. In this
study, we investigated whether low-dose radiation inhibit mediator release in human mast cells (HMC-
1(5C6) and LAD2) or allergic reaction in passive cutaneous anaphylaxis and late-phase cutaneous mouse
model. High dose radiation induced cell death but, low dose ionizing radiation of < 0.5 Gy did not induced
mast cell death. Low-dose ionizing radiation that did not induce cell death significantly suppressed
histamine release from human mast cells (HMC-1(5C6) and LAD2) activated by antigen-antibody reaction.
In order to reveal the inhibitory mechanism of mediator released by low dose ionizing radiation, we
examined the phosphorylation of intracellular signaling molecules such as Lyn, Syk, PLCγ, PKCs, and
2+
2+
2+
intracellular free Ca concentration ([Ca ]i). The phosphorylation of signaling molecules and [Ca ]i via
stimulation of FcεRI receptor was specifically inhibited by low dose ionizing radiation. In agreement with
its in vitro effect, ionizing radiation also significantly inhibited passive cutaneous anaphylaxis (PCA)
reaction and late-phase cutaneous response in anti-dinitrophenyl (DNP) IgE-sensitized mice. These results
indicate that ionizing radiation inhibits both mast cell-mediated immediate and delayed-type allergic
reactions in vivo and in vitro.
(PS5-32) A spreadsheet for detection of possible data fabrication in numerical data sets of the type
1
frequently encountered in cell and radiation biology survival studies. Helene Z. Hill, PhD and Joel H. Pitt,
1
2
2
PhD ; Rutgers NJ Medical School, Newark, NJ and Renaissance Associates, Princeton, NJ
The growing awareness of the frequency of falsified and fabricated data in scientific and medical
reports highlights the need for tools to identify problematic data prior to publication. Programs to detect
plagiarism are routinely used by a number of publishers and have led to a decrease in this type of
misconduct. Image manipulation can be detected by using programs such as one available on the Office
of Research Integrity website. Routine use of such programs will help to curtail this type of fraud, as well.
We have constructed a spreadsheet that can process numerical data sets of a type frequently encountered
in cell and radiation biology survival studies to detect possible data fabrication. Such detection is based
on the fact that fabrication may be indicated by the presence of unusual, unexpected patterns in data.
For example, rightmost terminal digits of counts taken from particle and scintillation counters should be
relatively uniform, whereas numbers invented by individuals do not generally have uniform terminal
digits. Hence, significant deviation from uniformity of the terminal digits of count data could be cause for
concern. Another suspect pattern, more specific to survival studies in radiation biology and pharmacology,
where samples are generally tested in triplicate, is an unusually high frequency of triples that include
303 | P a g e
