Page 59 - 2014 Printable Abstract Book
P. 59
(S1203) Normal tissue stem cell plasticity. Frank Pajonk, UCLA, Los Angeles, CA
(S1204) Functional importance of stem cells in the brain. Charles Limoli; Munjal Acahrya; Vipan Parihar;
Brianna Craver; Katherine Tran; Barrett Allen; and Vahan Martirosian, University of California, Irvine,
Irvine, CA
(S1205) Epithelial progenitor cells of the conducting airway and their response to ionizing radiation.
Barry Stripp, PhD, Cedars-Sinai Medical Center, Los Angeles, CA
Environmental, occupational or therapeutic exposure of lung tissue to ionizing radiation (IR)
contributes to tissue remodeling and an increase in cancer risk. Studies in other organs including the small
intestine have shown that exposure to IR has effects that differ between progenitor cell types, with
radiation-resistant progenitors contributing to tissue regeneration after sub-lethal exposure. However,
little is known of radiation effects on lung cells that may impact tissue homeostasis, remodeling and
cancer risk. We have employed in vivo genetic lineage tracing and cell fractionation approaches to define
cell-autonomous and microenvironmental factors that influence the proliferative and differentiation
potentials of distinct regional epithelial progenitor cells in response to IR exposure. The dependence of
progenitor cell responses to IR on Trp53 function was investigated using either germline or conditional
null mice. We have found that IR alters airway progenitor cell behavior in a cell type- and Trp53-dependent
fashion. We conclude that Trp53 regulates the replicative potential of airway epithelial progenitors for
tissue maintenance and repair. Supported by grants from NASA and CIRM.
S13 RADIOLOGICAL COUNTERMEASURES: TRANSLATIONAL EFFORTS USING LEUKOCYTE STEM CELL
FACTORS
The enhancements of the nation’s readiness to respond to potential radiological threats represent
high priority efforts by multiple federal departments. Highlighting these efforts there have been several
Food and Drug Administration (FDA) workshops and meetings; selected topics will be addressed in this
Symposium. Here we will focus on discussing use of leukocyte stem cell factors for medical management
of radiation casualties and will address: (1) the human experience for use of G-CSF, (2) animal-based
scientific evidence supporting FDA approval for use of G-CSF, (3) biological dosimetry approaches to
identify individuals with life-threatening radiation injury, and (4) national preparedness for medical
response to a radiologic/nuclear event.
(S1301) Status of Cytokines for Management of the Hematopoietic Sub-Syndrome of ARS in Accidentally
2
1
Irradiated Humans. Nicholas Dainiak ; Glen I. Reeves, Yale University School of Medicine, New Haven,
CT and ARA, Arlington, VA
2
1
Over 13 million people have received cytokine therapy to manage human disease in clinical
settings such as therapeutic radiotherapy, febrile neutropenia, dose-dense chemotherapy, stem cell
mobilization and myelodysplastic syndrome. In addition to their safety profile, the pathophysiological
mechanisms of both cytokine action and radiation-induced hematopoietic sub-syndrome (HS) are
reasonably well understood. Today, cytokines are considered standard therapy for victims of a nuclear
57 | P a g e
(S1204) Functional importance of stem cells in the brain. Charles Limoli; Munjal Acahrya; Vipan Parihar;
Brianna Craver; Katherine Tran; Barrett Allen; and Vahan Martirosian, University of California, Irvine,
Irvine, CA
(S1205) Epithelial progenitor cells of the conducting airway and their response to ionizing radiation.
Barry Stripp, PhD, Cedars-Sinai Medical Center, Los Angeles, CA
Environmental, occupational or therapeutic exposure of lung tissue to ionizing radiation (IR)
contributes to tissue remodeling and an increase in cancer risk. Studies in other organs including the small
intestine have shown that exposure to IR has effects that differ between progenitor cell types, with
radiation-resistant progenitors contributing to tissue regeneration after sub-lethal exposure. However,
little is known of radiation effects on lung cells that may impact tissue homeostasis, remodeling and
cancer risk. We have employed in vivo genetic lineage tracing and cell fractionation approaches to define
cell-autonomous and microenvironmental factors that influence the proliferative and differentiation
potentials of distinct regional epithelial progenitor cells in response to IR exposure. The dependence of
progenitor cell responses to IR on Trp53 function was investigated using either germline or conditional
null mice. We have found that IR alters airway progenitor cell behavior in a cell type- and Trp53-dependent
fashion. We conclude that Trp53 regulates the replicative potential of airway epithelial progenitors for
tissue maintenance and repair. Supported by grants from NASA and CIRM.
S13 RADIOLOGICAL COUNTERMEASURES: TRANSLATIONAL EFFORTS USING LEUKOCYTE STEM CELL
FACTORS
The enhancements of the nation’s readiness to respond to potential radiological threats represent
high priority efforts by multiple federal departments. Highlighting these efforts there have been several
Food and Drug Administration (FDA) workshops and meetings; selected topics will be addressed in this
Symposium. Here we will focus on discussing use of leukocyte stem cell factors for medical management
of radiation casualties and will address: (1) the human experience for use of G-CSF, (2) animal-based
scientific evidence supporting FDA approval for use of G-CSF, (3) biological dosimetry approaches to
identify individuals with life-threatening radiation injury, and (4) national preparedness for medical
response to a radiologic/nuclear event.
(S1301) Status of Cytokines for Management of the Hematopoietic Sub-Syndrome of ARS in Accidentally
2
1
Irradiated Humans. Nicholas Dainiak ; Glen I. Reeves, Yale University School of Medicine, New Haven,
CT and ARA, Arlington, VA
2
1
Over 13 million people have received cytokine therapy to manage human disease in clinical
settings such as therapeutic radiotherapy, febrile neutropenia, dose-dense chemotherapy, stem cell
mobilization and myelodysplastic syndrome. In addition to their safety profile, the pathophysiological
mechanisms of both cytokine action and radiation-induced hematopoietic sub-syndrome (HS) are
reasonably well understood. Today, cytokines are considered standard therapy for victims of a nuclear
57 | P a g e
