Page 19 - Amnio Breathe Hensler BioLabs 2020

invention belongs. Publications cited herein and the materials for which they are cited
are specifically incorporated by reference.

Those skilled in the art will recognize, or be able to ascertain using no more than routine
experimentation, many equivalents to the specific embodiments of the invention
described herein. Such equivalents are intended to be encompassed by the following
claims.

Claims

1. A method of treating or preventing a lung disorder or injury in a human, horse, dog or
cat comprising administering to the lung of the human, horse, dog or cat in need of
treatment a sterile amniotic fluid, in an amount effective to treat, alleviate, or prevent
one or more symptoms of the lung disorder or injury, wherein the amniotic fluid is devoid
of amniotic cells, micronized membrane, and chorion particles.

2. The method of claim 1 wherein the amniotic fluid is administered in a dosage unit
between about 0.1 cc and about 10.0 cc.

3. The method of claim 3 wherein the amniotic fluid is diluted with sterile water, saline or
buffer.

4. The method of claim 1 wherein the amniotic fluid is administered to the pulmonary or
nasal system.

5. The method of claim 1 wherein the amniotic fluid is administered in the form of
aerosol or with a nebulizer.

6. The method of claim 6 wherein the aerosol produces droplets having a size between
about 1.5 and 5 microns.

7. The method of claim 7 wherein at least 30% of the aerosol droplets have a size
between 1.5 and 5 microns in diameter.

8. The method of claim 6 wherein the amniotic fluid is administered via a nebulizer.

9. The method of claim 1 wherein the amniotic fluid is administered in combination with
one or more therapeutic, prophylactic or diagnostic agents.

10. The method of claim 10 wherein the amniotic fluid is administered in combination
with one or more agents selected from the group consisting of bronchodilators,
corticosteroids, methylxanthines, phosphodiesterase-4 inhibitors, anti-angiogenesis
agents, antimicrobial agents, antioxidants, anti-inflammatory agents, growth factors,
immunosuppressant agents, anti-allergic agents, and combinations thereof.

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