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1st Int. Transborder Conf. of the Timor Island: Timor %u2013 Science without borderDili, 7-8 May 2025175Identification of Secondary Metabolite, Bioactivity and Sunscreen Activity Test of ethanol Extract Centella asiatica LI G. M. N. Budiana*and Maria T. JelitaDeptartment of Chemistry, Faculty of Education and Teacher Training, Universitas Nusa Cendana, Indonesia.*Corresponding author: gusti_budiana@staf.undana.ac.idAbstractA study on the identification of secondary metabolite components, bioactivity, and sunscreen activity of ethanol extract from pegagan leaves (Centella asiatica L.) has been conducted. The aim of this research was to identify the classes of secondary metabolites present in the ethanol extract of pegagan leaves and to evaluate its bioactivity and sunscreen potential. The study involved several stages: extraction of pegagan leaf powder using the maceration method, bioactivity testing using the Brine Shrimp Lethality Test (BSLT), and sunscreen activity evaluation using ultraviolet (UV) spectroscopy. The results revealed that the ethanol extract of Centella asiatica leaves contains secondary metabolites from the flavonoid, tannin, saponin, and steroid groups. The lethal concentration (LC%u2085%u2080) value obtained was 69.051 ppm, which is below 1000 ppm, indicating that the extract is toxic to Artemia salina larvae. A lower LC%u2085%u2080 value suggests a stronger potential to eliminate disease-causing agents, such as bacteria and viruses. The sunscreen activity test showed that at a concentration of 250 ppm, the extract had a Sun Protection Factor (SPF) value of 9.33, which falls within the category of moderate to high protection.Keywords: identification, extract, secondary metabolite, sunscreen and bioactivity.