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1st Int. Transborder Conf. of the Timor Island: Timor %u2013 Science without borderDili, 7-8 May 202537Effect of Native Grass and Mucuna bracteata Proportions in Silage Production on the Content of Tannins, Saponins, and Anti-Trypsin Gagus S. H. Tatik; T. O. Dami Dato and M. L. Mullik*Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa CendanaJln. Adisucipto, Penfui, Kupang, Indonesia 85001*Corresponding author: marthenmullik@staf.undana.ac.idAbstractThis study aimed to determine the effect of different proportions of natural grass and Mucuna bracteata in silage production on the content of anti-nutritional factors: tannins, saponins, and anti-trypsin. The study used a Completely Randomized Design consisting of 3 treatments and 5 replications, resulting in a total of 15 experimental units. The treatments were mixture native grass and Mucuna bracteata at a ratio of 40%:60% (P1), 50%:50% (P2), 60%:40% (P3). The variables observed were the content of tannin,saponin, and anti-trypsin. The data were analyzed using analysis of variance to assess the effect of the treatments on the measured parameters. To determine differences between treatments, an LSD (Least Significant Difference) test was conducted. The results showed that the proportion of natural grass and Mucuna bracteata in silage production had no significant effect on the anti-nutritional contents of the silage. In treatment P1, tannin content was 12.19%, saponin content was 5.89%, and anti-trypsin content was 0.12%. In treatment P2, tannin content was 11.27%, saponin content was 5.50%, and anti-trypsin content was 0.13%. In treatment P3, tannin content was 11.87%, saponin content was 5.72%, and anti-trypsin content was 0.13%. These results indicate no significant differences among the treatments. It can be concluded that varying proportions of natural grass and Mucuna bracteata in silage do not significantly affect the levels of anti-nutritional compounds. However, the resulting silage still maintains good quality for use, suggesting that the presence of anti-nutritional factors does not compromise the feed%u2019s nutritional value.Keywords: anti-nutritional factors, Mucuna bracteata, natural grass, silage.