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1st Int. Transborder Conf. of the Timor Island: Timor %u2013 Science without borderDili, 7-8 May 202564Fusarium Wilt of Shallots in Semau Island, Kupang Regency: Pathogen Identification and Biocontrol Potential of Locally Isolated Trichoderma asperellumMayavira V. Hahuly *, Agnes V. Simamora, Petronella S. Nenotek, Lily F. Ishaq, Moresi Airthur, Yohanista ListraDepartment of Agrotechnology, Faculty of Agriculture, Universitas Nusa Cendana Jl. Adisucipto, Penfui, Kupang NTT, Indonesia.*E-mail: mayavira.hahuly@staf.undana.ac.idAbstractShallot (Allium cepa var. aggregatum L.) cultivation across Indonesia is severely threatened by Fusarium wilt (twisted disease), caused by the Fusarium oxysporumspecies complex (FOC). However, information on the presence and incidence of this disease in Kupang Regency, particularly on Semau Island, remains limited. This study aimed to assess the occurrence and disease incidence of Fusarium wilt in main shallotproducing District on Semau Island, and to isolate and evaluate the biocontrol potential of indigenous Trichoderma spp. against the disease. Disease incidence varied across surveyed sites. Molecular identification using ITS4/ITS5 primers confirmed the presence of multiple Fusarium species, including F. acutatum and F. longipes, complicating disease management efforts. Trichoderma isolates obtained from healthy shallot rhizospheres were identified as Trichoderma asperellum. In vitro dual culture assays demonstrated significant suppression of Fusarium pathogens, with the T. asperellum isolate T.UT3 achieving 33.66%u201356.33% inhibition across various Fusarium strains. Given the unsustainability of traditional chemical controls, these findings highlight native T. asperellum strains as promising biocontrol agents for integration into Integrated Disease Management (IDM) strategies. This approach offers a sustainable solution to mitigate Fusarium wilt, enhance soil health, and protect shallot production in Nusa Tenggara Timur. Future research should focus on field application and bioformulation development to optimize biocontrol efficacy at scale.Keywords: biocontrol, fusarium wilt, Integrated disease management, shallot, trichoderma asperellum