Page 11 - The order of draw day 2_UPDATED

DAY 2

Clinical Laboratory Standard Institute (CLSI) guidelines recommend an “Order of draw”
The order of draw is designed to eliminate the possibility of cross-contamination of chemicals.
ORDER OF ADDITIVE COMMON TEST PERFORMED LAB SECTION
DRAW Special Handling
YELLOW SPS: Sodium Blood cultures Microbiology
polyanetholesulfonate: Bacteremia, Septicemia Use a sterile cleansing
aids in the recovery of agent: Iodine or
microorganisms. Chlorohexidine
LIGHT Sodium citrate: prevents PT: Prothrombin Time Chemistry
BLUE coagulation by binding to APTT: Activated Partial This tube must be filled
calcium in the specimen. Thromboplastin Time to the top. 9:1 or it will
Sodium citrate is the PTT: Partial Thromboplastin Time be rejected from the lab.
anticoagulant used for BT: Bleeding Time
coagulation studies TT: Thrombin Time
because it preserves the FDP: Fibrinogen degradation
coagulation factors product
SERUM Thixotropic Gel / no MOST CHEMISTRY TEST Serology / Chemistry
chemical Allow to sit for 15-30
minutes to ensure
coagulation before
placing it in the
centrifuge.
GREEN / Heparin: combined with Ammonia (on ice) Chemistry
PST sodium, lithium, or carboxyhemoglobin & STAT
ammonium ion. Heparin electrolytes.
works by inhibiting
thrombin in the coagulation
cascade
LAVENDER EDTA: CBC: Complete Blood Count Hematology
ethylenediaminetetraacetic RBC: Red blood cell count Use a needle that does
acid. EDTA inhibits WBC white blood cell count not cause hemolysis.
coagulation by binding to Platelet count
calcium present in the H&H: Hemoglobin and Hematocrit
specimen ESR: Erythrocyte Sed Rate
GRAY Sodium fluoride FBS: fasting blood sugar Chemistry
Potassium oxalate GTT: Glucose tolerance test Blood alcohol testing
sodium fluoride- preserves Blood alcohol levels requires a legal form
glucose for 3days Lactic acid (on ice) called Chain of Custody,
potassium oxalate, prevents cleanse with something
clotting by binding calcium other than alcohol.