Page 16 - PHLEBOTOMY STUDY GUIDE

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Clinical Laboratory Standard Institute (CLSI) guidelines recommend an “Order of draw”
The order of draw is designed to eliminate the possibility of cross-contamination of chemicals.
ORDER OF ADDITIVE COMMON TEST PERFORMED LAB SECTION
DRAW Special Handling
YELLOW SPS: Sodium Blood cultures Microbiology
polyanetholesulfonate: Bacteremia, Septicemia Use a sterile cleansing
aids in the recovery of agent: Iodine or
microorganisms. Chlorohexidine
LIGHT Sodium citrate: prevents PT: Prothrombin Time Chemistry
BLUE coagulation by binding to APTT: Activated Partial This tube must be filled
calcium in the specimen. Thromboplastin Time to the top. 9:1 or it will
Sodium citrate is the PTT: Partial Thromboplastin Time be rejected from the lab.
anticoagulant used for BT: Bleeding Time
coagulation studies TT: Thrombin Time
because it preserves the FDP: Fibrinogen degradation
coagulation factors product
SERUM Thixotropic Gel / no MOST CHEMISTRY TEST Serology / Chemistry
chemical Allow to sit for 15-30
minutes to ensure
coagulation before
placing it in the
centrifuge.
GREEN / Heparin: combined with Ammonia (on ice) Chemistry
PST sodium, lithium, or carboxyhemoglobin & STAT
ammonium ion. Heparin electrolytes.
works by inhibiting
thrombin in the coagulation
cascade
LAVENDER EDTA: CBC: Complete Blood Count Hematology
ethylenediaminetetraacetic RBC: Red blood cell count Use a needle that does
acid. EDTA inhibits WBC white blood cell count not cause hemolysis.
coagulation by binding to Platelet count
calcium present in the H&H: Hemoglobin and Hematocrit
specimen ESR: Erythrocyte Sed Rate
GRAY Sodium fluoride FBS: fasting blood sugar Chemistry
Potassium oxalate GTT: Glucose tolerance test Blood alcohol testing
sodium fluoride- preserves Blood alcohol levels requires a legal form
glucose for 3days Lactic acid (on ice) called Chain of Custody,
potassium oxalate, prevents cleanse with something
clotting by binding calcium other than alcohol.
It is important for you, the phlebotomist to stay current with changes in your industry.

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