Page 142 - Ebook-Book JCMS 2025

Diagnostic Accuracy of QuantiFERON-TB Gold In-tube
Assay and Mucosal-associated Invariant T cells for Diagnosing
Tuberculous Spondylodiscitis
Korawish Mekariya1, Pisanukiet Saneewong Na Ayutthaya2, Akkaraporn Naowanirut3, Artit Wongsa4
,
Boonrat Tassaneetrithep4, Wiwit Tantibhedhyangkul5, Popchai Ngamskulrungroj6, Sorranart Muangsomboon7
,
Pipat Cheiwvit8, Sirichai Wilartratsami1, Panya Luksanapruksa1, Nasuda Danchaivijitr8, Kitidete Boonchai1
,
Apisit Rattanatanasarn1, Monchai Ruangchainikom1*
1 Department of Orthopaedic Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University
2 Department of Orthopaedic Surgery, Aranyaprathet Hospital
3 Department of Orthopaedic surgery, Hatyai Hospital
4 Center of Research Excellence in Immunoregulation, Faculty of Medicine, Siriraj Hospital, Mahidol University
5 Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University
6 Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University
7 Department of Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University
8 Department of Radiology, Faculty of Medicine, Siriraj Hospital, Mahidol University
*Corresponding Author E-mail: monchai.ortho@gmail.com
Background: Methods: Abstract
Tuberculous spondylodiscitis (TS) is a serious form of tuberculosis (TB) that requires tissue
biopsy for definitive diagnosis. However, this can delay appropriate treatment, potentially
resulting in long-term disability. Immunodiagnostic approach, such as interferon-gamma
release assays (IGRAs), offers promising alternatives for TS diagnosis. In addition,
recent advancements suggest that mucosal-associated invariant T (MAIT) cells may serve as
biomarkers for TB infection. We aim to evaluate the diagnostic performance of IGRA using
the QuantiFERON-TB Gold In-Tube test (QFT-GIT) and MAIT cell analysis for diagnosing
TS.
Patients with clinically and radiologically suspected spondylodiscitis were prospectively
enrolled. All underwent IGRA test using QFT-GIT. Peripheral blood mononuclear cells were
isolated and stained for surface antigens to identify MAIT cells, defined as CD3+CD161+
Vα7.2+T cells. MAIT cells population including—CD4+, CD8+, and double-negative
(CD4+CD8+) were analyzed. Receiver operating characteristic analysis was performed to
determine cutoff value and discriminative ability for TS diagnosis
140 Joint Conference in Medical Sciences 2025