Page 153 - Ebook-Book JCMS 2025

Adipose-derived Mesenchymal Stem Cells for Chronic Wound
Healing: A Preliminary Study in a Cat as an Animal Model for
Human Application
Natthima Suwan1, Sasipat Teerawongsuwan2, Ruttachuk Rungsiwiwut2*
1 Faculty of Medicine, Srinakharinwirot University
2 Department of Anatomy, Faculty of Medicine, Srinakharinwirot University
*Corresponding Author E-mail: ruttachuk@g.swu.ac.th
Background: Methods: Results: Abstract
Chronic wounds are a significant clinical challenge in both human and veterinary medicine,
often resulting in delayed healing and reduced quality of life. Adipose-derived mesenchymal
stem cells (ADMSCs) provide regenerative properties. This preliminary study investigates
the isolation, characterization, and application on chronic wounds in cats to establish a
translational implication for humans.
Subcutaneous adipose tissue was collected from three healthy female cats during surgical
neutralization at Wipawadee Veterinary Clinic. The tissues were processed at the Stem
Cell Laboratory, Department of Anatomy, Faculty of Medicine, Srinakharinwirot University.
ADMSCs were isolated by enzymatic digestion and cultured in MEM supplemented
with 10% fetal bovine serum and 5 ng/ml basic fibroblast growth factor. ADMSCs
characterization included morphology, surface antigen expression, and trilineage
differentiation (osteogenic, chondrogenic, and adipogenic). Bacterial toxins and Mycoplasma
contamination tested negative. A 12-year-old neutered male mixed-breed domestic
cat presented with a non-healing chronic wound on the skull with comorbid positive
feline immunodeficiency virus and impaired kidney function and was selected for treatment.
The wound was resistant to standard antibiotics. Allogenic ADMSCs were administered
subcutaneously surrounding the wound margins monthly for three months. Wound
dimensions were monitored daily during routine wound care. The cat was supplemented
with protein.
The isolated ADMSCs displayed a fibroblast-like morphology with surface adherence. Flow
cytometry supported the surface antigens of mesenchymal stem cells: positive expression
of CD44, CD90, and CD105 and negative expression of CD34 and MHC-II. The cells
demonstrated the ability to differentiate into three lineages. No endotoxin or mycoplasma
contamination was detected. The clinical wound showed improvement after two weeks
following the first injection, with a stable reduction of wound size to closure in five months.
Conclusion: This case demonstrated the potential effectiveness of ADMSCs as a therapeutic approach
for chronic wound management in cats, with possible translational implications for human.
Harmony in health: Innovation for Sustainable Medicine
151