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Formulation Optimization and in Vitro Release of a Topical
Cream Containing Hispidulin and Nepetin for Atopic Dermatitis
Premrutai Thitilertdecha1, Suphitcha Limjiasahapong2, Poonsin Poungpairoj1, Theeraporn Maneesawat1
,
Varangkana Tantithavorn1, Panida Kethip3, Siriphan Manocheewa2, Nattawat Onlamoon1
,
Yongyut Sirivatanauksorn2,4, Sakda Khoomrung 2,4,5,6,7*
1 Siriraj Research Group in Immunobiology and Therapeutic Sciences, Research Department, Faculty of
Medicine, Siriraj Hospital, Mahidol University
2 Siriraj Metabolomics and Phenomics Center, Faculty of Medicine, Siriraj Hospital, Mahidol University
3 Department of Pharmacy General Pharmaceutical Production Division, Faculty of Medicine, Siriraj
Hospital, Mahidol University
4 Thailand Metabolomics Association
5 Siriraj Center of Research Excellence in Metabolomics and System biology (SiCORE-MSB), Faculty of
Medicine, Siriraj Hospital, Mahidol University
6 Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University
7 Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University
*Corresponding Author E-mail: sakda.kho@mahidol.ac.th
Background: Abstract
Long-term use of topical corticosteroids for treating atopic dermatitis is well known for its
unavoidable side effects, highlighting the need for effective alternatives with lower toxicity.
Naturally-occurring flavonoids, such as hispidulin and nepetin, have been shown to
possess immunosuppressive properties and exhibit synergistic effects. This study thus
aimed to develop a novel topical formulation incorporating both compounds to achieve
immunosuppressive doses.
Objective: An oil-in-water cream and lotion containing hispidulin and nepetin at a ratio of 1:3 were
formulated. Franz diffusion cells were employed to evaluate release profiles of both
compounds from different formulations to identify the optimum one. The most suitable
synthetic membrane was selected for in vitro release testing and a fully validated ultra-
performance liquid chromatography coupled with a tandem mass spectrometry (UPLC-MS/
MS) was used to quantify the compounds.
90 Joint Conference in Medical Sciences 2025