Page 93 - Ebook-Book JCMS 2025

Results: The polyvinylidene fluoride membrane demonstrated superior diffusion properties
compared to cellulose acetate, mixed cellulose ester, nylon, and polyestersulfone. The
UPLC-MS/MS method exhibited high accuracy with linearity of > 0.9980 and a recovery
rate above 85%. Both hispidulin and nepetin permeated within 15 min and were effectively
delivered over 24 hours. Of all formulations tested, the cream containing 0.1% hispidulin
and 0.3% nepetin showed the highest release rates at 12.2% and 17.4%, respectively. These
released amounts exceeded previously reported effective immunosuppression doses of
66.7 μM hispidulin and 200 μM nepetin.
Conclusion: The optimized cream effectively released both compounds at the therapeutic levels within 6
hours while maintaining good stability. These findings then suggest the promising topical
alternative to corticosteroids for immunosuppressive therapy.
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