Page 231 - ebook HCC

GENEVA, SWITZERLANDA, SWITZERLAND
PROGRAMME AND ABSTRACTSAND ABSTRACTS
EASL
228 PROGRAMME GENEV EASL HCC SUMMITHCC SUMMIT 229
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229
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
FEBRUAR
Poster Board Number C21
URINARY PROTON NUCLEAR MAGNETIC
RESONANCE SPECTROSCOPY IDENTIFIES
ALTERED METABOLIC PATHWAYS IN
HEPATOCELLULAR CARCINOMA

Nimzing G. Ladep on behalf of PROLIFICA; Anthony Dona ,
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Shahid Khan , Edith Okeke , Maud Lemoine , Ramou Njie , John Chetwood ,
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Duguru Mary , Deborah Garside , Haddy Fye , Mary Crossey , Mark Thursz ,
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Elaine Holmes , Simon D. Taylor-Robinson 1
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1 Medicine, Imperial College London, London, United Kingdom, Jos University Teaching
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Hospital, Jos, Nigeria, Medical Research Council, Fajara, The Gambia
Corresponding author’s e-mail: n.ladep@imperial.ac.uk
Introduction: Metabonomics offer opportunities to appraise global outcomes of metabolic
changes and which can provide insights to altered pathways in hepatocellular carcinoma
(HCC).
Aims: To identify altered HCC pathways using urinary proton nuclear magnetic resonance
spectroscopy (NMR) of patients from Africa.
Methodology: Urine samples were collected at two sites in West Africa on the case-
control platform of the “Prevention of Liver Fibrosis and Carcinoma in Africa” (PROLIFICA)
study. 600MHz NMR spectrometer was used to acquire one-dimensional spectral data
from the urine samples. The initial test sample from 202 subjects were validated by a
CLINICAL POSTER ABSTRACTS were confirmed using statistical total correlation spectroscopy (STOCSY) tool, as well as CLINICAL POSTER ABSTRACTS
second cohort, comprising 463 subjects [141 with HCC, 56 with cirrhosis (Cir), 178 with
non-cirrhotic liver disease (DC) and 88 healthy volunteers (NC )]. Identified metabolites
by comparing urinary spectra with corresponding reference standards.
Results: Multivariate modelling of the spectral data showed distinct urinary metabolic
profiles of patients with HCC, compared to Cir, DC and NC with high sensitivity and
specificity. Several metabolites were significantly altered (p<0.0001) in urine of HCC
patients, compared to non-HCC liver disease controls. Using the Kyoto Encyclopaedia of
Genes and Genomes (KEGG) pathway mapping tool, we identified several pathways that
could be altered in HCC.

Conclusions: Urinary NMR of the studied population has identified metabolites that were
perturbed in HCC, suggesting alteration of several pathways requiring further trawling.