Page 310 - ebook HCC

GENEVA, SWITZERLANDA, SWITZERLAND
PROGRAMME
EASL HCC SUMMITHCC SUMMIT
308
308 PROGRAMME AND ABSTRACTSAND ABSTRACTS GENEV EASL 309
309
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
FEBRUAR
Poster Board Number C74
STUDY OF MULTIDRUG RESISTANCE IN
HEPATOCELLULAR CARCINOMA: THE ROLE OF
NUCLEAR MEDICINE

Ana F. Brito , Mónica Mendes , Marina Ribeiro , Ana M. Abrantes , Results: Hep3B2.1-7 cell line is one that has higher levels of uptake and retention of
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Ana C. Gonçalves , Ana B. Sarmento-Ribeiro , Francisco Castro Sousa 1.2 4 , 18 F-FDG and also 99m Tc-MIBI. The HepG2 cell line has a lower uptake and retention and a

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José G. Tralhão 1 2 4 , Maria F. Botelho 1 2 higher expression of MRP1. The levels of Pgp and LRP expression are similar for all cell
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1 Biophysics Unit, Faculty of Medicine, University of Coimbra, Center of Investigation on lines. Through studies of modulation was verified, by incubating the cells with verapamil, a
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Environmental, Genetics and Oncobiology (CIMAGO), Applied Molecular Biology and considerable increase in F-FDG and 99m Tc-MIBI retention in all cell lines.
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Hematology Group, Faculty of Medicine, University of Coimbra, Surgical Department,
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Surgery A, HUC, Coimbra, Portugal Conclusion: There is an inverse relationship between MRP1 expressions and uptake and
retention of 99m Tc-MIBI and F-FDG. Through modulation studies it was found that Pgp
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Corresponding author’s e-mail: anabrito816@gmail.com has an active role on MDR phenomenon in HCC. The uptake and retention profiles for the
two radiopharmaceuticals are similar, showing that the F-FDG can be used to study the
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action of MDR proteins in HCC cells, presented as an alternative to 99m Tc-MIBI.
Introduction: Surgical resection and liver transplantation offer the best chance of a
cure for Hepatocellular Carcinoma (HCC). However, only less than 15% of patients are
candidates to these therapies and are treated with other therapies such as chemotherapy.
However HCC is known to be highly resistant to chemotherapy, which is due in part to
overexpression of multidrug resistance proteins (MDR).
Aims: A method to measure the function of these proteins involves the study of
radiolabeled substrate 99m Tc-MIBI uptake. Studies have demonstratedthat F-FDG uptake
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is associated with tumor differentiation and the expression of MDR proteins in HCC. Tthis
study aims evaluate and compare the uptake and retention of F-FDG and 99m Tc-MIBI in
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three human HCC cell lines with different expression levels of p53 and to correlate with the
CLINICAL POSTER ABSTRACTS Methodology: Human HCC cell lines used were HepG2 (wp53), HuH7(mp53) and CLINICAL POSTER ABSTRACTS
expression of three MDR proteins (Pgp, MRP1 and LRP).
Hep3B2.1-7(p53null). Cell suspensions with 2x10 cells/ml were incubated with 25µCi/ml
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Tc-MIBI. Samples of 200μl were collected at different periods of time
of F-FDG or
99m
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which were centrifuged separating the supernatant from the pellet. Activity was measured
Tc-MIBI retention was obtained by incubating the cell
in a well counter. F-FDG and
99m
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suspension with radioisotope during 60 minutes. Thereafter, the cells were centrifuged
and medium renewed. The following procedure was similar to the uptake studies. The
proteins levels of Pgp, MRP1 and LRP were determined by flow cytometry. To evaluate
MDR modulation, retention studies were performed in the presence of verapamil (Pgp
inhibitor) prior to incubation with F-FDG.
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