Page 327 - ebook HCC

EASL HCC SUMMITHCC SUMMIT
PROGRAMME
GENEV
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324 PROGRAMME AND ABSTRACTSAND ABSTRACTS GENEVA, SWITZERLANDA, SWITZERLAND EASL 325
324
FEBRUAR
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
Poster Board Number C85
INTRATUMORAL INJECTION OF S-NITROSO-N- Results: Residual HCC and hepatocholangiocarcinoma were observed in all samples of
ACETYLCYSTEINE (SNAC) IN A RODENT MODEL animal treated with saline solution and none necrosis focus were observed in this group.
Residual HCC and hepatocholangiocarcinoma with necrosis were evident in 66.6% (2 of
OF NON-ALCOHOLIC STEATOHEPATITIS (NASH)- 3 animals) of ethanol group and similarly in the SNAC group 66.6% (4 of 6 animals) with
RELATED HEPATOCELLULAR CARCINOMA (HCC) residual HCC and hepatocholangiocarcinoma. The difference between SNAC and alcohol
was that alcohol caused more complete necrosis.

Conclusion: This model replicates the major stages of NASH including cirrhosis and HCC
Jose Tadeu Stefano , Mariana M. Torres, Isabel V. A. Pereira, Bruno Cogliati , and could be used in novel therapeutic approaches. Besides, these results suggest that
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Marcelo G. Oliveira , Flair J. Carrilho , Claudia P. Oliveira 1 the SNAC offers a new form of anti-HCC therapy.
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1 São Paulo Clínicas Liver Cancer Group, Hospital das Clínicas, Instituto do Câncer
2
do Estado de São Paulo, University of São Paulo School of Medicine, Department of
Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo,
São Paulo, Institute of Chemistry, University of Campinas (UNICAMP),
3
Campinas, SP, Brazil
Corresponding author’s e-mail: cpm@usp.br
Introduction: Hepatocellular carcinoma (HCC) is a well-recognized complication
of advanced Nonalcoholic steatohepatitis (NASH). Previous studies by our group
demonstrated in vitro that S-nitroso-N-acetylcysteine (SNAC), an NO donor, has an
antiproliferative effect. However, the in vivo effect of SNAC over NASH-related HCC has
not been addressed.
Background: We developed a rat model that reproduces NASH with cirrhosis and HCC
and evaluated the effectiveness of SNAC intratumoral injection in this animal model in
comparison with alcohol and saline solution.
CLINICAL POSTER ABSTRACTS deficient high fat diet (35% total fat, 54% trans fatty acid enriched) and simultaneously CLINICAL POSTER ABSTRACTS
Methodology: Adult Sprague-Dawley rats, weighing 250-300g, were fed a choline-

exposed to diethylnitrosamine (13-15 mg/day) in drinking water during 16 weeks to induce
NASH, cirrhosis and HCC. B-mode and Doppler ultrasonography was performed weekly
in these experimental rats. Based in our previous studies, the animals with NASH that
developed focal liver lesions with suggestive malignancy were underwent elastography
and contrast-enhanced ultrasonography. Tissue stiffness of the nodules on elastography
were classified in negative (elastic strain) or positive (hard and no strain) comparing with
surrounding liver parenchyma. Contrasted study classified focal lesions according to type
of enhancement and wash out. After imaging diagnostic of HCC the animals were treated
with one of 0.1 ml of intratumoral injection of ethanol (n=3), saline solution (n=3) and
SNAC (n=6; 2500µM). After 72 hours of the experiment, autopsies were done to collect
liver samples for morphological and histological examination.