Page 338 - ebook HCC

EASL HCC SUMMITHCC SUMMIT
PROGRAMME AND ABSTRACTSAND ABSTRACTS
GENEVA, SWITZERLANDA, SWITZERLAND
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336 PROGRAMME GENEV EASL 337
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
FEBRUAR
Poster Board Number C93
EFFECT OF LEPTIN ADMINISTRATION
ON ETHANOL INDUCED APOPTOSIS
AND FIBROGENESIS IN THE MOUSE
HEPATOCELLULAR CARCINOMA CELL LINES

Balasubramaniyan Vairappan , Murugaiyan Gopal , In addition, ethanol significantly increased the reactive oxygen species (ROS) and the
1
2
Ramachandra R. Bhonde , Nalini Namasivayam 1 expressions of mRNA of caspase-3 (2 fold), procollagen type I (about 24 fold), MMP
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1 Biochemistry and Biotechnology, Annamalai University, Chidambaram, 2 (3.8 fold), MMP 9 (2.5 fold) and TIMP-1 (1.3 fold) as compared to untreated control
2 Biotechnology, National Center for Cell Science, Pune, India mouse HCC cell lines. Leptin coadministration significantly down regulated the mRNA
expressions of caspase-3 (3 fold), procollagen type I (3 fold), MMP 2 (2 fold), MMP 9
Corresponding author’s e-mail: balamaniyan@gmail.com (4.5 fold) and TIMP-1 (10 fold) as compared to that of ethanol alone treated mouse HCC
cell lines.
Introduction: Obesity is associated with hepatocellular carcinoma (HCC). Leptin, an Conclusion: Thus, our experimental data provide evidence above the potential
anti-obesity hormone exerts potent modulatory properties both in vivo and in vitro. protective effect of leptin on ethanol elicited damage in the mouse HCC cell lines,
warranting population based and mechanistic studies.
Aims: We have previously shown that reduction of hepatotoxicity with leptin in vivo. The
aim of this study was to evaluate the effect of leptin on ethanol induced fibrogenesis and
apoptosis in mouse hepatocellular carcinoma (HCC) cell lines.

Methodology: In vitro, 48 h after treatment with or without leptin (31.2nM), ethanol (500
mM) and leptin+ethanol to mouse HCC cell lines. Results were statistically evaluated
using one way analysis of variance (ANOVA) followed by Duncan_s Multiple Range Test
(DMRT).
CLINICAL POSTER ABSTRACTS dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (P<0.05) and trypan blue CLINICAL POSTER ABSTRACTS
Results: Ethanol exposure significantly reduced the cell viability as evidenced by 3-(4,5
dye exclusion method (TBE) (P<0.05). Moreover, ethanol treated cells significantly
lowered thymidine incorporation (P<0.05) and increased DNA fragmentation. Ethanol
incubations also significantly increased the % of apoptotic cells (P<0.05). These results
were compared with that of untreated control cell lines. Leptin cotreatment with ethanol
showed significantly raised (P<0.05) cell viability, DNA synthesis and significantly
(P<0.05) decreased apoptotic cells and DNA ladder formation.