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Live-Cell Analysis Handbook — Third Edition
Reproducible, quantitative data to conduct
pharmacological analysis
To demonstrate the pharmacological utility of the single spheroid
assay, we performed a comparison of three different drugs using
3D spheroids derived from the SKOV-3 ovarian cancer line (Figure
3). The data shows a concentration-dependent inhibitory growth
effect for all compounds, and illustrates how compound potencies
can be directly compared within the same assay.
CMP CIS OXA Vehicle
µM
Figure 3. Effect of CMP, cisplatin (CIS) and oxaliplatin (OXA) on growth of SKOV-3 cells in a 3D spheroid assay. SKOV-3 cells were plated at a density
of 5,000 cells per well and spheroid allowed to form (72 h). Cells were then treated with serial compound dilutions and kinetics of spheroid growth and
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shrinkage were obtained. Plate graph shows the individual well Largest Brightfield area (μm ) over time. IC50 curves represent the Largest Brightfield area
(μm ) at 204 h post-treatment. Data were collected over 240 h period at 6 h intervals. Each data point represents mean ±SEM, n=8.
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