Page 74 - ASME_NEMB_2016_Program

Technical Program TRACK 6

used a model drug, Diclofenac (DS) a nonsteroidal anti-inflammatory drug plied in overcoming the multiple barriers encountered in drug delivery and
(NSAID), which is commonly utilized in the treatment of arthritic disorders, to the employment of cell-like microparticles as tools to investigate the various
study the drug release characteristics by using this nanoparticle. We first de- phenomena in biological interactions.
veloped DS encapsulated CS coated PLGA nanoparticle and use in vitro set
up to study drug release properties. PLGA nanoparticles were synthesized
by double- emulsion solvent evaporation technique. The resulted nanoparti- Polyvinylpyrrolidone Coating of Ironoxide nanoparticles as mag-
cles were evaluated based on their particle size, zeta potential, entrapment netic drug delivery agents
efficacy, FTIR, and effect of pH in drug release study. About 400 nm of aver-
age diameters and uniform morphology of the particles were confirmed by Poster Presentation. NEMB2016-6150
scanning electron microscope (SEM) imaging and dynamic light scattering
(DLS) measurement. Chitosan coating over PLGA surface was confirmed by
FTIR study and positive zeta potential value for coated sample. Drug entrap- Yavuz Işçi, M. Gökçe Bekaroğlu, Sevim Işçi, Istanbul Technical
ment efficacy was up to 60%. Chitosan coated sample showed a pH respon- University, Istanbul, Select State/Province,Turkey
sive drug release in in-vitro. The release was about 60% more at pH5.5 than
at pH7.4. The results of our study indicated the development of chitosan Iron-oxide particles recently are researched for the potential applications of
coating over PLGA nanoparticle for pH dependent controlled release DS targeted drug delivery due to the magnetic properties. The surfaces of the
drug for therapeutic applications. iron-oxide particles must be modified to reduce the toxicity and to load the
drug to the particles. Biopolymers are good surface modifier of the colloidal
particles such as iron-oxide particles. The degree of surface coverage of the
Object Shape and Elasticity Impact Interactions in the Tumor Mi- colloidal iron-oxide particles affects the stability, toxicity, magnetic proper-
croenvironment ties and drug loading efficiency. In this study, the interactions of iron-oxide
(Fe O ) particles and PVP were determined with the colloidal properties.
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The proper concentration of PVP for the whole coverage of the iron-oxide
Poster Presentation. NEMB2016-6146 particles was found for the possible magnetic drug delivery applications by
controlling the colloidal properties of the dispersions. Magnetic properties
Jenolyn F. Alexander, Houston Methodist Research Institute, and toxicity of the fully covered bioiron-oxide was also determined for the
Houston, TX, United States, Veronika Kozlovskaya, Jun Chen, Uni- possible applications.
versity of Alabama at Birmingham, Birmingham, AL, United States,
Thomas Kuncewicz, Biana Godin, Eugenia Kharlampieva, Hous-
ton Methodist Research Institute, Houston, TX, United States Colloidal Properties and in-Vitro Evaluation of Hydroxyl Ethylene
Cellulose Coated Iron Oxide Nanoparticles for Targeted Drug
Cancer chemotherapy necessitates the contrivance of a therapeutic system Delivery
to successfully negotiate biobarriers and efficiently deliver therapeutics to
the diseased cells without affecting healthy sites. In Nature, blood-borne Poster Presentation. NEMB2016-6151
objects like erythrocytes, leukocytes and platelets possess non-spherical
shape and elasticity due to which they exhibit various circulatory patterns, M. Gökçe Bekaroğlu, Yavuz Işçi, Sevim Işçi, Istanbul Technical
biological interactions as well as the ability to extravasate through fenes-
trations in the vascular endothelium, in response to stimuli in the tumor University, Istanbul,Turkey
microenvironment. We hypothesize that systemically administered parti-
cles with cell-like physical attributes such as geometry and elasticity can In this study, superparamagnetic iron oxide (Fe O ) nanoparticles were pre-
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likewise, marginate through vasculature, extravasate through endothelial pared for the targeted drug delivery applications by controlling the colloidal
fenestrations that are much smaller than their dimensions, and interact with properties with cellulosic polymer, Hydroxyethyl cellulose (HEC). Fe O par-
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the cells in the tumor microenvironment. For this purpose, we engineered ticles were treated with HEC in a variable range of polymer concentration.
biocompatible, Elastic Microparticles (EM), which are “soft” hollow polymeric Rheological, electrokinetical, magnetorheological and morphological prop-
microcapsules (shells) with spherical and cubical (edge-possessing) shapes erties of the dispersions were investigated to have stable and fully covered
by layer-by-layer (LbL) assembly of hydrogen-bonded tannic acid/ poly(N-vi- surfaces of Fe O particles by coating with HEC and to obtain non-toxic
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nylpyrrolidone)/ (TA/PVPON) polymers on sacrificial templates. We compared biocompatible multifunctional magnetic particles. The fully HEC covered
the 2 micron-sized spherical and cubical shells with their rigid counterparts iron-oxide particles were thermally, magnetically characterized and tested
displaying the same surface properties - core shells or Rigid Microparticles for toxicity. The cancer drugs, DOX, were adsorbed on the particles. The
(RM), in their interaction with cells present in the tumor microenvironment effects of these particles on the cancer cells were examined to report a nan-
namely, macrophage (J774A.1), endothelial (human microvascular endothe- odrug system, which is potentially open up new possibilities in the design of
lial, HMVEC) and breast cancer cells (4T1, MDA-MB-231 and SUM159). The therapeutic agents.
hollow elastic shells are unrecognized by macrophage cells in contrast
to the rigid core-shells that are internalized within 15 minutes of contact,
whereas there is no significant difference between the uptake of spherical Engineering Gold Nanoconstructs for Targeted Delivery and
and cubical core-shells or shells. Cubical shells are internalized 5-fold more Controlled Release of Antibiotics
efficiently by human microvascular endothelial cells in static conditions and
2.5 and 6 fold more efficiently by breast cancer cells (SUM159 and MDA- Poster Presentation. NEMB2016-6068
MB-231, respectively). At physiological flow conditions similar to the tumor
vasculature, shear rate of 100 s-1, the cubical objects interact substantially
more than spherical objects, but at a normal vascular shear rate of 10 s-1, Jingyi Chen, Jenkins Samir, Emily K. Miller, University of Arkan-
shape does not seem to play a significant role. We also explored the ex- sas, Fayetteville, AR, United States, Daniel Meeker, Mark Smeltzer,
travasation potential of the polymeric systems by passing them through a University of Arkansas for Medical Sciences, Little Rock, AR, United
nylon membrane with 0.8 micron-sized pores, resembling the fenestrations States
in the tumor endothelium and found that the EM are capable of squeezing
thorough pores one-third their dimension unlike RM that were retained on Drug delivery systems with targeted capability and on-demand controlled
the membrane. release mechanism are particularly appealing for designing optimal med-
ications in many disease treatments. Controlled release systems for drug
74 Our study demonstrates that cubical shape favours the interaction with delivery using nanocarriers have been developed and studied for more
breast cancer cells and elasticity prevents recognition by immune cells. Our than three decades. Gold nanostructures have been used as drug delivery
study signifies how the various mechanisms occurring in nature can be ap- vehicles in chemotherapy because of their biocompatibility, facile surface