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Isolation and Characterization of Diazotrophic Bacteria ........ Sugarcane   55



                     with  all  other  treatments  except  T 4   (14.38  %).  T 4  produced  statistically  similar  brix  (%)
                     followed by T 3  (15.48%) and T 2  (15.18%). The highest pol percent cane was also found in
                     T 5  (10.84%) which was statistically similar with all other treatment except T 4  (9.51%). The
                                                                   -1
                     highest yield of cane was obtained in T 5  (95.31 tha ), which was statistically similar with
                                                             -1
                     all other treatments except control (61.16 tha ). Hari (1995) reported that inoculation of N
                     fixing  microbes  to  sugarcane  have  increased  the  cane  yield  by  5-15%,  saved  25  kg
                                 -1
                     fertilizer N ha  and also improved the juice quality parameter viz; sucrose and purity.

                     Interaction effect of variety × treatment
                            Due  to  interaction  of  sugarcane  variety  and  different  treatments  all  growth
                     parameters  influenced  significantly  (Table  2,  Figure  3).  Highest  germination  (%)  was
                     found in interaction  V 1 T 4  (74.67 %), which was statistically similar with  interaction V 1 T 2
                     (71.78 %), V 1 T 3  (71.57 %), V 1 T 5  (70.19 %) and V 1 T 6  (71.51 %). The highest number of
                                                                   3
                                                                       -1
                     tiller  was  obtained  in  interaction  V 2 T 3   (152.09  ×10   ha )  which  was  statistically  similar
                                                   -1
                                                                         -1
                                               3
                                                                     3
                                                                                           3
                                                                                               -1
                     with that of V 2 T 2  (149.81 × 10  ha ), V 2 T 5  (149.26 × 10  ha ), V 2 T 6  (147.04 ×10  ha ) and
                                       3
                                           -1
                     V 2 T 4   (144.07  ×  10   ha ).  The  highest  number  of  millable  canes  was  obtained  in
                                                   3
                                                       -1
                     interaction  of  V 2 T 4   (100.51  ×  10   ha )  which  was  statistically  at  par  with  that  of  V 2 T 3
                                                       -1
                                                    3
                                                                                              3
                                                                            -1
                                                                         3
                                  -1
                                                                                                  -1
                               3
                     (97.93 ×10  ha ), V 2 T 2  (96.97 × 10  ha ), V 2 T 5  (96.79 ×10  ha ),  V 1 T 5  (96.04 × 10  ha ),
                                                                                3
                                                                                   -1
                                                          3
                                                             -1
                                       -1
                                   3
                     V 1 T 4  (95.20 ×10  ha ), V 1 T 3  (94.641 × 10  ha ),  V 2 T 6  (94.53 × 10  ha )  and V 1 T 6  (93.99
                             -1
                         3
                     × 10  ha ). The highest pol percent cane was obtained in interaction of V 2 T 5  (12.47 %)
                     which  was  statistically  at  par  with  that  of  interactions  V 2 T 3   (11.97  %),  V 2 T 1   (11.96  %),
                     V 2 T 2   (11.86  %),  V 2 T 6   (11.85  %)  and  V 2 T 4   (11.48  %).  The  highest  yield  of  cane  was
                                                           -1
                     obtained  in  interaction  of  V 1 T 5   (99.26  tha )  which  was  statistically  at  par  with  that  of
                                                               -1
                                                                                 -1
                                              -1
                                                                                                  -1
                     interactions V 1 T 4  (95.43 tha ), V 1 T 2  (95.33 tha ), V 1 T 6  (94.42 tha ), V 1 T 3  (91.68 tha ),
                                                                            -1
                                    -1
                                                      -1
                     V 2 T 5   (91.36  tha ),  V 2 T 4   (90.93  tha )  and  V 2 T 6   (87.79  tha ).  Chauhan  et  al.  (2010)
                     supported  our  result  where  they  reported  that  inoculation  of  diazotroph
                     (Gluconacetobacter)  to  sugarcane  significantly  increased  the  plant  height,  cane  length,
                     number of millable canes resulting the cane yield increase by 42% over control.

                                  Figure 3. Performance of diazotrophic bacteria in sugarcane
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