Page 85 - BJS vol. 35

Response of 2, 4-D on Callus Induction and Plantlet ........ Sugarcane 77

Figure 2. A. Regenerated plantlets were transplanted in the polybag and then
hardening shade, B. Somaclone in the field

Table 1. Effects of 2, 4-D on callus induction in Sugarcane
Callus induction Days required of
Treatments Weight (gm)/Callus
(%) callus initiation
-1
1 mg l 2, 4-D 76.67 b 12.00 1.43 c
-1
2 mg l 2, 4-D 80.00 b 12.33 1.63 b
-1
3 mg l 2, 4-D 100.00 a 12.67 1.77 a
-1
4 mg l 2, 4-D 96.67 a 13.67 1.63 b
-1
5 mg l 2, 4-D 76.67 b 13.67 1.37 c
LSD (0.05) 5.538 - 0.1238
Figure in the column with same letter do not differ significantly at 5% level of probability
as per DMRT
Table 2. Effects of 2, 4-D on shoots and roots formation in sugarcane

Callus % Days required
Number of
Treatments produced shoot/callus of shoot Number of
root/callus
shoot initiation
-1
1 mg l 2, 4-D 96.67 a 7.00 a 21.33 d 4.00 a
-1
2 mg l 2, 4-D 76.67 b 7.00 a 21.67 d 2.00 b
-1
3 mg l 2, 4-D 76.67 b 5.67 b 23.33 c 1.33 c
-1
4 mg l 2, 4-D 53.33 c 4.00 c 24.33 b 0.00 d
-1
5 mg l 2, 4-D 23.33 d 1.33 d 35.67 a 0.00 d
LSD (0.05) 9.592 0.599 0.5538 0.4533
Figure in the column with same letter do not differ significantly at 5% level of probability
as per DMRT